Impacts of Blended Bombyx mori Silk Fibroin and Recombinant Spider Silk Fibroin Hydrogels on Cell Growth

Polymers

Jittima Amie Luckanagul¹  Siriporn Damrongsakkul¹  Juthamas Ratanavaraporn¹  Sorada Kanokpanont¹  Chavee Laomeephol¹  Thomas Scheibel²  Martin Humenik²  Apichai Vasuratna³ 

[1] Biomaterial Engineering for Medical and Health Research Unit, Faculty of Engineering, Chulalongkorn University, Bangkok 10330, Thailand;Department of Biomaterials, Faculty of Engineering Science, University of Bayreuth, Prof.-Rüdiger-Bormann Str. 1, 95447 Bayreuth, Germany;Department of Obstetrics and Gynecology, Faculty of Medicine, Chulalongkorn University, Bangkok 10330, Thailand;

关键词: silk fibroin;    spider silk;    hydrogel;    self-assembly;    cell culture;   

DOI  :  10.3390/polym13234182

来源: DOAJ

【 摘 要 】

Binary-blended hydrogels fabricated from Bombyx mori silk fibroin (SF) and recombinant spider silk protein eADF4(C16) were developed and investigated concerning gelation and cellular interactions in vitro. With an increasing concentration of eADF4(C16), the gelation time of SF was shortened from typically one week to less than 48 h depending on the blending ratio. The biological tests with primary cells and two cell lines revealed that the cells cannot adhere and preferably formed cell aggregates on eADF4(C16) hydrogels, due to the polyanionic properties of eADF4(C16). Mixing SF in the blends ameliorated the cellular activities, as the proliferation of L929 fibroblasts and SaOS-2 osteoblast-like cells increased with an increase of SF content. The blended SF:eADF4(C16) hydrogels attained the advantages as well as overcame the limitations of each individual material, underlining the utilization of the hydrogels in several biomedical applications.

【 授权许可】

Unknown