Biophysical and Biological Tools to Better Characterize the Stability, Safety and Efficacy of a Cosmeceutical for Acne-Prone Skin

Molecules

Nicola Zerbinati¹  Stefania Guida²  Pier Luca Bencini³  Raffaele Rauso⁴  Roberto Mocchi⁵  Giulia Grimaldi⁵  Maria Chiara Capillo⁵  Elsa Liga⁵  Cristina Maccario⁵  Sabrina Sommatis⁵ 

[1] Department of Medicine and Surgery, University of Insubria, 21100 Varese, Italy;Dermatology Unit, Department of Surgical, Medical, Dental and Morphological Sciences Related to Transplant, Oncology and Regenerative Medicine, University of Modena and Reggio Emilia, 41124 Modena, Italy;Istituto di Chirurgia e Laser-Chirurgia in Dermatologia (I.C.L.I.D.), 20121 Milan, Italy;Maxillofacial Surgery Unit, Department of Medicine and Surgery, University of Campania “Luigi Vanvitelli”, 80138 Naples, Italy;UB-CARE S.r.l.-Spin-Off University of Pavia, 27100 Pavia, Italy;

关键词: acne-prone skin;    Cutibacterium acnes;    rheological properties;    keratinocytes;    human reconstructed 3D models;    biofilm;   

DOI  :  10.3390/molecules27041255

来源: DOAJ

【 摘 要 】

(1) Background: Acne is a widespread skin disease, especially among adolescents. Following the COVID-19 pandemic and the use of masks, the problem has been affecting a greater number of people, and the attention of the skin care beauty routine cosmetics has been focused on the “Maskne”, caused by the sebum excretion rate (SER) that stimulates microbial proliferation. (2) Methods: the present study was focused on the rheological characterization and quality assurance of the preservative system of an anti-acne serum. The biological effectiveness (cytotoxicity—skin and eye irritation—antimicrobial, biofilm eradication and anti-inflammatory activity) was evaluated in a monolayer cell line of keratinocytes (HaCaT) and on 3D models (reconstructed human epidermis, RHE and human reconstructed corneal epithelium, HCE). The Cutibacterium acnes, as the most relevant acne-inducing bacterium, is chosen as a pro-inflammatory stimulus and to evaluate the antimicrobial activity of the serum. (3) Results and Conclusions: Rheology allows to simulate serum behavior at rest, extrusion and application, so the serum could be defined as having a solid-like behavior and being pseudoplastic. The preservative system is in compliance with the criteria of the reference standard. Biological effectiveness evaluation shows non-cytotoxic and irritant behavior with a good antimicrobial and anti-inflammatory activity of the formulation, supporting the effectiveness of the serum for acne-prone skin treatment.

【 授权许可】

Unknown