Suppressive Effect of Two Cucurbitane-Type Triterpenoids from Momordica charantia on Cutibacterium acnes-Induced Inflammatory Responses in Human THP-1 Monocytic Cell and Mouse Models

Molecules

Chi-I Chang¹  Lu-Te Chuang²  Hsiang Chang²  Tsung-Hsien Tsai³  Po-Jung Tsai⁴  Wen-Cheng Huang⁴  Jong-Ho Chyuan⁵  Yu-Chen Hou⁶ 

[1] Department of Biological Science and Technology, National Pingtung University of Science and Technology, Pingtung 912, Taiwan;Department of Biotechnology and Pharmaceutical Technology, Yuanpei University of Medical Technology, Hsinchu 300, Taiwan;Department of Dermatology, Taipei Municipal Wan Fang Hospital and Taipei Medical University, Taipei 116, Taiwan;Department of Human Development and Family Studies, National Taiwan Normal University, Taipei 106, Taiwan;Hualien District Agricultural Research and Extension Station, Hualien 973, Taiwan;Master Program in Food Safety, College of Nutrition, Taipei Medical University, Taipei 110, Taiwan;

关键词: acne;    Cutibacterium acnes;    Momordica charantia;    anti-inflammation;    kuguacin R;    3β,7β,25-trihydroxycucurbita-5,23-dien-19-al (TCD);   

DOI  :  10.3390/molecules26030579

来源: DOAJ

【 摘 要 】

Cutibacterium acnes (formerly Propionibacterium acnes) is one of the major bacterial species responsible for acne vulgaris. Numerous bioactive compounds from Momordica charantia Linn. var. abbreviata Ser. have been isolated and examined for many years. In this study, we evaluated the suppressive effect of two cucurbitane-type triterpenoids, 5β,19-epoxycucurbita-6,23-dien-3β,19,25-triol (Kuguacin R; KR) and 3β,7β,25-trihydroxycucurbita-5,23-dien-19-al (TCD) on live C. acnes-stimulated in vitro and in vivo inflammatory responses. Using human THP-1 monocytes, KR or TCD suppressed C. acnes-induced production of interleukin (IL)-1β, IL-6 and IL-8 at least above 56% or 45%, as well as gene expression of these three pro-inflammatory cytokines. However, a significantly strong inhibitory effect on production and expression of tumor necrosis factor (TNF)-α was not observed. Both cucurbitanes inhibited C. acnes-induced activation of the myeloid differentiation primary response 88 (MyD88) (up to 62%) and mitogen-activated protein kinases (MAPK) (at least 36%). Furthermore, TCD suppressed the expression of pro-caspase-1 and cleaved caspase-1 (p10). In a separate study, KR or TCD decreased C. acnes-stimulated mouse ear edema by ear thickness (20% or 14%), and reduced IL-1β-expressing leukocytes and neutrophils in mouse ears. We demonstrated that KR and TCD are potential anti-inflammatory agents for modulating C. acnes-induced inflammation in vitro and in vivo.

【 授权许可】

Unknown