Morpho-Physiological Testing of NaCl Sensitivity of Tobacco Plants Overexpressing Choline Oxidase Gene

Plants

GalinaN. Raldugina¹  SergeyV. Evsukov¹  LiliyaR. Bogoutdinova²  EkaterinaN. Baranova²  AlexanderA. Gulevich³ 

[1] K. A. Timiryazev Institute of Plant Physiology, Russian Academy of Sciences, Botanicheskaya Street 35, 127276 Moscow, Russia;Plant Cell Biology Laboratory, All-Russia Research Institute of Agricultural Biotechnology, Russian Academy of Sciences, Timiryazevskaya Street 42, 127550 Moscow, Russia;Plant Cell Engineering Laboratory, All-Russia Research Institute of Agricultural Biotechnology, Timiryazevskaya 42, 127550 Moscow, Russia;

关键词: salinity;    glycine betaine;    Nicotiana tabacum L.;    codA gene;    transgenic plants;    osmolytes;   

DOI  :  10.3390/plants10061102

来源: DOAJ

【 摘 要 】

In this study the transgenic lines (TLs) of tobacco (Nicotianatabacum L.), which overexpress the heterologous gene encoding the bacterial enzyme choline oxidase were evaluated. The goal of our work is to study the effect of choline oxidase gene expression on the sensitivity of plant tissues to the action of NaCl. The regenerative capacity, rhizogenesis, the amount of photosynthetic pigments and osmotically active compounds (proline and glycine betaine) were assessed by in vitro cell culture methods using biochemical and morphological parameters. Transgenic lines with confirmed expression were characterized by high regeneration capacity from callus in the presence of 200 mmol NaCl, partial retention of viability at 400 mmol NaCl. These data correlated with the implicit response of regenerants and whole plants to the harmful effects of salinity. They turned out to be less sensitive to the presence of 200 mmol NaCl in the cultivation medium, in contrast to the WT plants.

【 授权许可】

Unknown