Coating-Dependent Effects of Silver Nanoparticles on Tobacco Seed Germination and Early Growth

International Journal of Molecular Sciences

DanielMark Lyons¹  Renata Biba²  Petra Cvjetko²  PetraPeharec Štefanić²  Dubravko Pavoković²  Dajana Matić²  Mirta Tkalec²  Biljana Balen²  Ilse Letofsky-Papst³ 

[1] Center for Marine Research, Ruđer Bošković Institute, G. Paliaga 5, 52210 Rovinj, Croatia;Department of Biology, Faculty of Science, University of Zagreb, Horvatovac 102a, 10000 Zagreb, Croatia;Institute of Electron Microscopy and Nanoanalysis (FELMI), Graz University of Technology, Graz Centre for Electron Microscopy (ZFE), Austrian Cooperative Research (ACR), Steyrergasse 17, 8010 Graz, Austria;

关键词: silver nanoparticles;    AgNP stability;    Nicotiana tabacum L.;    germination;    root length;    fresh and dry weight;   

DOI  :  10.3390/ijms21103441

来源: DOAJ

【 摘 要 】

Silver nanoparticles (AgNPs) are used in a wide range of consumer products because of their excellent antimicrobial properties. AgNPs released into the environment are prone to transformations such as aggregation, oxidation, or dissolution so they are often stabilised by coatings that affect their physico-chemical properties and change their effect on living organisms. In this study we investigated the stability of polyvinylpyrrolidone (PVP) and cetyltrimethylammonium bromide (CTAB) coated AgNPs in an exposure medium, as well as their effect on tobacco germination and early growth. AgNP-CTAB was found to be more stable in the solid Murashige and Skoog (MS) medium compared to AgNP-PVP. The uptake and accumulation of silver in seedlings was equally efficient after exposure to both types of AgNPs. However, AgNP-PVP induced only mild toxicity on seedlings growth, while AgNP-CTAB caused severe negative effects on all parameters, even compared to AgNO₃. Moreover, CTAB coating itself exerted negative effects on growth. Cysteine addition generally alleviated AgNP-PVP-induced negative effects, while it failed to improve germination and growth parameters after exposure to AgNP-CTAB. These results suggest that the toxic effects of AgNP-PVP are mainly a consequence of release of Ag⁺ ions, while phytotoxicity of AgNP-CTAB can rather be ascribed to surface coating itself.

【 授权许可】

Unknown