Yeast HXK2 gene reverts glucose regulation mutation of penicillin biosynthesis in P. chrysogenum

Brazilian Journal of Microbiology

Ana T. Marcos¹  Juan F. Martín¹  Francisco J. Fernández²  Armando Mejía²  Edmundo A. Pérez²  Javier Barrios-González²  Francisco Fierro² 

[1] Instituto de Biotecnología de León;Universidad Autónoma Metropolitana;

关键词: glucose regulation;    glucokinase;    penicillin;    Penicillium chrysogenum;   

DOI  :  10.1590/S1517-83822014000300017

来源: DOAJ

【 摘 要 】

The mutant Penicillium chrysogenum strain dogR5, derived from strain AS-P-78, does not respond to glucose regulation of penicillin biosynthesis and β-galactosidase, and is partially deficient in D-glucose phosphorilating activity. We have transformed strain dogR5 with the (hexokinase) hxk2 gene from Saccharomyces cerevisiae. Transformants recovered glucose control of penicillin biosynthesis in different degrees, and acquired a hexokinase (fructose phosphorylating) activity absent in strains AS- P-78 and dogR5. Interestingly, they also recovered glucose regulation of β-galactosidase. On the other hand, glucokinase activity was affected in different ways in the transformants; one of which showed a lower activity than the parental dogR5, but normal glucose regulation of penicillin biosynthesis. Our results show that Penicillium chrysogenum AS-P-78 and dogR5 strains lack hexokinase, and suggest that an enzyme with glucokinase activity is involved in glucose regulation of penicillin biosynthesis and β-galactosidase, thus signaling glucose in both primary and secondary metabolism; however, catalytic and signaling activities seem to be independent.

【 授权许可】

Unknown