Brazilian Journal of Microbiology | |
Yeast HXK2 gene reverts glucose regulation mutation of penicillin biosynthesis in P. chrysogenum | |
Edmundo A. Pérez2  Francisco J. Fernández2  Francisco Fierro2  Armando Mejía2  Ana T. Marcos1  Juan F. Martín1  Javier Barrios-gonzález2  | |
[1] ,Universidad Autónoma Metropolitana Departamento de Biotecnología Laboratorio de Ingeniería Genética y Metabolitos Secundarios Mexico D.F. ,Mexico | |
关键词: glucose regulation; glucokinase; penicillin; Penicillium chrysogenum; | |
DOI : 10.1590/S1517-83822014000300017 | |
来源: SciELO | |
【 摘 要 】
The mutant Penicillium chrysogenum strain dogR5, derived from strain AS-P-78, does not respond to glucose regulation of penicillin biosynthesis and β-galactosidase, and is partially deficient in D-glucose phosphorilating activity. We have transformed strain dogR5 with the (hexokinase) hxk2 gene from Saccharomyces cerevisiae. Transformants recovered glucose control of penicillin biosynthesis in different degrees, and acquired a hexokinase (fructose phosphorylating) activity absent in strains AS- P-78 and dogR5. Interestingly, they also recovered glucose regulation of β-galactosidase. On the other hand, glucokinase activity was affected in different ways in the transformants; one of which showed a lower activity than the parental dogR5, but normal glucose regulation of penicillin biosynthesis. Our results show that Penicillium chrysogenum AS-P-78 and dogR5 strains lack hexokinase, and suggest that an enzyme with glucokinase activity is involved in glucose regulation of penicillin biosynthesis and β-galactosidase, thus signaling glucose in both primary and secondary metabolism; however, catalytic and signaling activities seem to be independent.
【 授权许可】
CC BY-NC
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