Evidence from phylogenetic and genome fingerprinting analyses suggests rapidly changing variation in Halorubrum and Haloarcula populations

Frontiers in Microbiology

Adit eNaor¹  Nikhil eRam Mohan²  Ryan W Wheeler²  Matthew S Fullmer²  R Thane ePapke²  Andrea M Makkay²  Johann Peter eGogarten²  Antonio eVentosa³ 

[1] Tel Aviv University;University of Connecticut;University of Seville;

关键词: MLSA;    halobacteria;    genome fingerprinting;    Aran-Bidgol Lake;    environmental population;   

DOI  :  10.3389/fmicb.2014.00143

来源: DOAJ

【 摘 要 】

Halobacteria require high NaCl concentrations for growth and are the dominant inhabitants of hypersaline environments above 15% NaCl. They are well documented to be highly recombinogenic, both in frequency and in the range of exchange partners.In this study, we examine the genetic and genomic variation of cultured, naturally co-occurring environmental populations of Halobacteria.Sequence data from multiple loci (~2500bp) identified closely related strains belonging to the genera Halorubrum and Haloarcula. Genome fingerprinting using a random priming PCR amplification method to analyze these isolates revealed diverse banding patterns within and across each of the genera and surprisingly even for isolates that are identical at the nucleotide level for five protein coding sequenced loci. This variance in genome structure even between identical multilocus sequence analysis (MLSA) haplotypes suggests that accumulation of variation is rapid, perhaps occurring every generation.

【 授权许可】

Unknown