Halovirus HF2 Intergenic Repeat Sequences Carry Promoters

Viruses

Friedhelm Pfeiffer¹  Mike Dyall-Smith¹  Brendan Russ² 

[1] Computational Biology Group, Max-Planck-Institute of Biochemistry, 82152 Martinsried, Germany;Department of Microbiology, Clayton Campus, Monash University, Clayton 3800, Australia;

关键词: halovirus;    haloarchaea;    halobacteria;    Halorubrum coriense;    transcription;    promoter;   

DOI  :  10.3390/v13122388

来源: DOAJ

【 摘 要 】

Halovirus HF2 was the first member of the Haloferacalesvirus genus to have its genome fully sequenced, which revealed two classes of intergenic repeat (IR) sequences: class I repeats of 58 bp in length, and class II repeats of 29 bp in length. Both classes of repeat contain AT-rich motifs that were conjectured to represent promoters. In the present study, nine IRs were cloned upstream of the bgaH reporter gene, and all displayed promoter activity, providing experimental evidence for the previous conjecture. Comparative genomics showed that IR sequences and their relative genomic positions were strongly conserved among other members of the same virus genus. The transcription of HF2 was also examined by the reverse-transcriptase-PCR (RT-PCR) method, which demonstrated very long transcripts were produced that together covered most of the genome, and from both strands. The presence of long counter transcripts suggests a regulatory role or possibly unrecognized coding potential.

【 授权许可】

Unknown