Acetylation of cell wall is required for structural integrity of the leaf surface and exerts a global impact on plant stress responses

Frontiers in Plant Science

Daniel J Kliebenstein²  Henrik Vibe Scheller⁴  Susanna eAtwell⁵  Maria eStranne⁶  Helle Juel Martens⁶  Sara Fasmer Hansen⁶  Yumiko eSakuragi⁶  Pai Rosager Pedas⁶  Lorenzo eFimognari⁶  Majse eNafisi⁶  Christiane eNawrath⁷ 

[1] Copenhagen Plant Science Center;DNRF Center DynaMO;Laurence Berkeley National Laboratory;University of California at Berkeley;University of California at Davis;University of Copenhagen;University of Lausanne;

关键词: Epidermis;    Microscopy, Electron;    Peroxidase;    trichomes;    Botrytis cinerea;    cuticles;   

DOI  :  10.3389/fpls.2015.00550

来源: DOAJ

【 摘 要 】

The epidermis on leaves protects plants from pathogen invasion and provides a waterproof barrier. It consists of a layer of cells that is surrounded by thick cell walls, which are partially impregnated by highly hydrophobic cuticular components. We show that the Arabidopsis T-DNA insertion mutants of REDUCED WALL ACETYLATION 2 (rwa2), previously identified as having reduced O-acetylation of both pectins and hemicelluloses, exhibit pleiotrophic phenotype on the leaf surface.The cuticle layer appeared diffused and was significantly thicker and underneath cell wall layer was interspersed with electron-dense deposits. A large number of trichomes were collapsed and surface permeability of the leaves was enhanced in rwa2 as compared to the wild type. A massive reprogramming of the transcriptome was observed in rwa2 as compared to the wild type, including a coordinated up-regulation of genes involved in responses to abiotic stress, particularly detoxification of reactive oxygen species, and defense against microbial pathogens (e.g. lipid transfer proteins, peroxidases). In accordance, peroxidase activities were found to be elevated in rwa2 as compared to the wild type. These results indicate that cell wall acetylation is essential for maintaining the structural integrity of leaf epidermis, and that reduction of cell wall acetylation leads to global stress responses in Arabidopsis.

【 授权许可】

Unknown