Spectroscopic analysis and cytotoxic activity of quas-sinoid isolated from the seeds of Brucea javanicaon Hela cell

Indonesian Journal of Pharmacy

Jumina¹  Dwiprahasto I²  Mustofa²  Sonlimar Mangunsong² 

[1] Faculty of Mathematics and Natural Sciences, Department of Chemistry Universitas Gadjah Mada;Faculty of Medicine Universitas Gadjah Mada;

关键词: Quassinoid;    B. javanica;    Brusein A;    Spectroscopic;    Anticancer;   

DOI  :  http://dx.doi.org/10.14499/indonesianjpharm0iss0pp137-143

来源: DOAJ

【 摘 要 】

ThequassinoidsfromBruceajavanica exhibitbiologicalactivitiesas antimalarial,antitumor,antiviralandantiamoebicactivities.Thesequassinoids havingapromisingantitumoractivity.Thisresearch wasaimedtoisolated quassinoid(bruceineA,MW522g/mol) compoundfromB.javanica andto investigatethecytotoxicityofbruceineAunderinvitro conditionsinHelacells. Thecytotoxicityassays using MTTassay.Thebruceine showedagood cytotoxicactivityinHela cellsat24hourswithdoxorubicineasapositive control.Structuralelucidationof bruceine isdoneby usingUVandFT-IR spectra,NMRspectra recordedonTeslaav600,(400 MHz) 1HNMRinpyridine (D5),someinGyro(300MHz) 1HNMRinacetone-(d6),DMSO-(d6)andCdCl3 ,13CNMR,75MHz,massspectraonbiospectrometryLC-MS.AnalyticalHPLC retention time,tRwas 4.617 minutes (Met/H20) 60:40. Its Molecular formula was determinedtobe(C26 H34O11)bythe[M+H]+ ionpeakatm/z523 andm/z 1045 confirmed by High Resolution mass spectra Orbitrap (HRESIMS). From the results demonstrated that could be bruceine A compound refers to our spectral data.Itshas activityinIC50 value191,5µMonHelaCellsunderinvitro conditions.

【 授权许可】

Unknown