Careful with That Axe, Gene, Genome Perturbation after a PEG-Mediated Protoplast Transformation in Fusarium verticillioides

Toxins

Valeria Scala¹  Alessandro Grottoli²  Marzia Beccaccioli²  Massimo Reverberi²  Corrado Fanelli²  Chiara Dall’Asta³  Paola Battilani⁴  Riccardo Aiese Cigliano⁵  Walter Sanseverino⁵  Irantzu Anzar⁵ 

[1] Consiglio per la Ricerca in Agricoltura e l’Analisi dell’Economia Agraria - Research Center for Plant Protection and Certification, 00156 Roma, Italy;Department Environmental Biology, Università Sapienza, 00185 Roma, Italy;Department Food Chemistry, Università di Parma, 43120 Parma, Italy;Department Sustainable Crop Production, Università Cattolica del Sacro Cuore, 29100 Piacenza, Italy;Sequentia-Biotech SL, 08028 Barcelona, Spain;

关键词: gene deletion;    parasexual cycle;    in vitro evolution;    mitotic recombination;    bioinformatics;    Fusarium database;   

DOI  :  10.3390/toxins9060183

来源: DOAJ

【 摘 要 】

Fusarium verticillioides causes ear rot disease in maize and its contamination with fumonisins, mycotoxins harmful for humans and livestock. Lipids, and their oxidized forms, may drive the fate of this disease. In a previous study, we have explored the role of oxylipins in this interaction by deleting by standard transformation procedures a linoleate diol synthase-coding gene, lds1, in F. verticillioides. A profound phenotypic diversity in the mutants generated has prompted us to investigate more deeply the whole genome of two lds1-deleted strains. Bioinformatics analyses pinpoint significant differences in the genome sequences emerged between the wild type and the lds1-mutants further than those trivially attributable to the deletion of the lds1 locus, such as single nucleotide polymorphisms, small deletion/insertion polymorphisms and structural variations. Results suggest that the effect of a (theoretically) punctual transformation event might have enhanced the natural mechanisms of genomic variability and that transformation practices, commonly used in the reverse genetics of fungi, may potentially be responsible for unexpected, stochastic and henceforth off-target rearrangements throughout the genome.

【 授权许可】

Unknown