Pulsed field gel electrophoresis reveals chromosome length and number differences in Brazilian strains of Metarhizium Anisopliae

Brazilian Archives of Biology and Technology

Vanessa Kava-cordeiro²  Marisa Vieira De Queiroz¹  Aline Aparecida Pizzirani-kleiner¹  João Lúcio Azevedo¹ 

[1] ,Universidade Federal do Paraná Departamento de Genética Laboratório de Genética de MicrorganismosCuritiba PR ,Brazil

关键词: Metarhizium anisopliae;    pulsed-field gel electrophoresis;    chromosome polymorphisms;    parasexual cycle;    entomopathogenic fungus;   

DOI  :  10.1590/S1516-89132005000100001

来源: SciELO

【 摘 要 】

Electrophoretic karyotypes of eight wild-type strains of Metarhizium anisopliae var. anisopliae were obtained by pulsed-field gel electrophoresis. These strains were isolated from insects of six different Brazilian states. The chromosomal DNA molecules of three strains were separated into seven bands and of five strains into eight bands. Chromosome length polymorphisms were also observed. The size of the chromosomal DNA of all strains varied between 7.7 and 0.9 Mb using the Aspergillus nidulans chromosomes as size standards. The total genome size of these strains was estimated in at least 29.7 Mb. Some correlations between differences in karyotype and occurrence of parasexual cycle likewise the host specificity were discussed.

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