Identification and verification of key taste components in wampee using widely targeted metabolomics

Food Chemistry: X

Jian-bang Ji¹  Zhou-wei Duan²  Hui Xie³  Rong-hu Zhang³  Zhe Chen³  Qing-chun Yin³  Fu-chu Hu³  Hao Deng⁴ 

[1] Hainan Institute for Food Control / Key Laboratory of Tropical Fruits and Vegetables Quality Safety for State Market Regulation, Haikou 570311, China;Sanya Institute of Hainan Academy of Agricultural Sciences, Sanya, 572019, China;Institute of Agro-products Processing and Design, Hainan Academy of Agricultural Sciences / Key Laboratory of Tropical Fruit and Vegetable Cold-chain of Hainan Province, Haikou 570100, China;Institute of Tropical Fruit Trees, Hainan Academy of Agricultural Sciences / Key Laboratory of Tropical Fruit Tree Biology of Hainan Province, Haikou 570100, China;

关键词: Wampee;    Sweet taste;    Sweet–sour taste;    Cultivar;    Metabolomics;   

DOI  :  

来源: DOAJ

【 摘 要 】

Due to the lack of comprehensive evaluation of all metabolites in wampee, the metabolic reasons for taste differences are unclear. Here, two local varieties YF1 (sweet taste) and YF2 (sweet–sour taste), were selected for quality analysis, followed by ultra-performance liquid chromatography–tandem mass spectrometry (UPLC–MS/MS) based widely targeted metabolomic analysis. YF1 and YF2 were clearly separated by principal component analysis (PCA) and cluster analysis, and 449 metabolites were different between the cultivars, including 29 carbohydrates and 29 organic acids. Among them, d-galactose, d-mannose, and d-fructose 6-phosphate contributed mainly to the sweet taste of the YF1 wampee. l-citramalic acid, 2-hydroxyglutaric acid, and 3-methylmalic acid were the dominant organic acids in YF2 wampee, and therefore, contributed primarily to the sweet–sour taste. The differential metabolites were significantly enriched in the “ascorbate and aldarate metabolism” and “C5-branched dibasic acid metabolism” pathways. Ascorbate played a crucial role in the regulation of sugars and organic acids through those pathways. In addition, high-performance liquid chromatography (HPLC) based quantitative verification exhibited the same specific cultivar variations.

【 授权许可】

Unknown