L-Arginine/NO Pathway Metabolites in Colorectal Cancer: Relevance as Disease Biomarkers and Predictors of Adverse Clinical Outcomes Following Surgery

Journal of Clinical Medicine

Katarzyna Neubauer¹  Iwona Bednarz-Misa²  Agnieszka Kubiak²  Małgorzata Krzystek-Korpacka²  MariuszG. Fleszar²  Wojciech Witkiewicz³  Marek Zawadzki³  Bartosz Kapturkiewicz⁴ 

[1] Department of Gastroenterology and Hepatology, Wroclaw Medical University, 50-556 Wrocław, Poland;Department of Medical Biochemistry, Wroclaw Medical University, 50-368 Wrocław, Poland;Department of Oncological Surgery, Regional Specialist Hospital, 51-124 Wrocław, Poland;First Department of Oncological Surgery of Lower Silesian Oncology Center, 53-413 Wrocław, Poland;

关键词: asymmetric dimethylarginine (ADMA);    symmetric dimethylarginine (SDMA);    L-citrulline;    dimethylamine (DMA);    biomarker;    robotic surgery;   

DOI  :  10.3390/jcm9061782

来源: DOAJ

【 摘 要 】

The L-Arginine/NO pathway is involved in carcinogenesis and immunity. Its diagnostic and prognostic value in colorectal cancer (CRC) was determined using tandem mass spectrometry in 199 individuals (137 with CRC) and, during a three-day follow up, in 60 patients undergoing colorectal surgery. Citrulline was decreased and asymmetric (ADMA) and symmetric (SDMA) dimethylarginines and dimethylamine (DMA) were increased in CRC. The DMA increase corresponded with CRC advancement while arginine, ADMA, and SDMA levels were higher in left-sided cancers. Arginine, citrulline, ADMA, and DMA dropped and SDMA increased post incision. Females experienced a more substantial drop in arginine. The arginine and ADMA dynamics depended on blood loss. The initial SDMA increase was higher in patients requiring transfusions. Postoperative dynamics in arginine and dimethylarginines differed in robot-assisted and open surgery. Concomitant SDMA, citrulline, and DMA quantification displayed a 92% accuracy in detecting CRC. Monitoring changes in arginine, ADMA, and SDMA in the early postoperative period predicted postoperative ileus with 84% and surgical site infections with 90% accuracy. Changes in ADMA predicted operative morbidity with 90% and anastomotic leakage with 77% accuracy. If positively validated, L-arginine/NO pathway metabolites may facilitate CRC screening and surveillance, support differential diagnosis, and assist in clinical decision-making regarding patients recovering from colorectal surgery.

【 授权许可】

Unknown