Nodosilinea hunanesis sp. nov. (Prochlorotrichaceae, Synechococcales) from a Freshwater Pond in China Based on a Polyphasic Approach

Diversity

Shuheng Li¹  Fangfang Cai¹  Hang Zhang²  Gongliang Yu³  Renhui Li⁴ 

[1] Hubei Key Laboratory of Animal Nutrition and Feed Science, Hubei Collaborative Innovation Center for Animal Nutrition and Feed Safety, Wuhan Polytechnic University, Wuhan 430023, China;Hubei Water Resources Research Institute, Hubei Water Resources and Hydropower Science and Technology Information Center, Wuhan 430070, China;Key Laboratory of Algal Biology, State Key Laboratory of Freshwater Ecology and Biotechnology of China, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China;School of Life and Environmental Sciences, Wenzhou University, Wenzhou 325035, China;

关键词: 16S rRNA gene;    16S–23S ITS;    polyphasic approach;    Nodosilinea;   

DOI  :  10.3390/d14050364

来源: DOAJ

【 摘 要 】

A cyanobacterial strain (ZJJ01), morphologically identified as a Leptolyngbya-like species was isolated from a freshwater pond in Zhangjiajie National Forest Park, China, and it was characterized through a polyphasic study based on morphological, ecological, and molecular data. Morphologically, the strain fits the description of Leptolyngbya well, but under further examination, it formed a distinctive structure, namely nodules, which confirmed that it belonged to the genus of Nodosilinea. The 16S rRNA gene threshold value and 16S rRNA phylogenetic analyses clearly confirmed that the studied strain belongs to the genus Nodosilinea but that it is phylogenetically distinct from the 10 other species of Nodosilinea. Furthermore, The D1–D1’ and Box–B helix of the 16S–23S ITS region of the strain ZJJ01 were also different from those of previously described Nodosilinea species. On the basis of this polyphasic approach, here, we provide a description of the new taxon: Nodosilinea hunanesis sp. nov.

【 授权许可】

Unknown