A compact pulse-modulation cold air plasma jet for the inactivation of chronic wound bacteria: development and characterization

Heliyon

Apiwat Wijaikhum¹  Jomkhwan Meerak²  Athipong Ngamjarurojana²  Sureeporn Sarapirom³  Chakkrapong Kuensaen⁴  Phuthidhorn Thana⁵  Pipath Poramapijitwat⁶  Dheerawan Boonyawan⁷ 

[1] Corresponding author.;Department of Biology, Faculty of Science, Chiang Mai University, Chiang Mai, 50200, Thailand;Department of Physics and Materials Science, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand;Nanoscience and Nanotechnology, Faculty of Science, Maejo University, Chiang Mai, 50290, Thailand;PhD Degree Program in Applied Physics, Department of Physics and Materials Science, Faculty of Science, Chiang Mai University, Chiang Mai, 50200, Thailand;Plasma and Beam Physics Research Facility, Faculty of Science, Chiang Mai University, Chiang Mai, 50200, Thailand;ThEP Center, 239 Huay Kaew Road, Muang District, Chiang Mai, 50200, Thailand;

关键词: Biomedical engineering;    Plasma physics;    Cold atmospheric pressure plasmas;    Chronic wound bacteria;    Bactericidal action;   

DOI  :  

来源: DOAJ

【 摘 要 】

A compact low-temperature plasma jet device was developed to use ambient air as plasma gas. The device was driven by a 2.52-kV high-voltage direct-current pulse in a burst mode, with a repetition rate of 2 kHz. The maximum plasma discharge current was 3.5 A, with an approximately 10 ns full-width half-maximum. Nitric oxide, hydroxyl radical, atomic oxygen, ozone, and hydrogen peroxide—important reactive oxygen and nitrogen species (RONS)—were mainly produced. The amount of plasma-generated RONS can be controlled by varying the pulse-modulation factors. After optimization, the plasma plume length was approximately 5 mm and the treatment temperature was less than 40 °C. The preliminary bactericidal effects were tested on Staphylococcus aureus, Pseudomonas aeruginosa, and methicillin-resistant S. aureus (MRSA), and their biofilms. The results showed that the plasma can effectively inactivate S. aureus, P. aeruginosa, and MRSA in both time- and pulse-dependent manner. Thus, this produced plasma device proved to be an efficient tool for inactivating deteriorating bacteria. Further versatile utilization of this portable plasma generator is also promising.

【 授权许可】

Unknown