High Fat Diet Dysbiotic Mechanism of Decreased Gingival Blood Flow

Frontiers in Physiology

Nevena Jeremic²  Akash George³  Suravi Majumder³  Dragana Stanisic³  Mahavir Singh³  Sathnur Pushpakumar³  Suresh C. Tyagi³ 

[1] Department of Dentistry, Faculty of Medical Sciences, University of Kragujevac, Kragujevac, Serbia;Department of Pharmacy, Faculty of Medical Sciences, University of Kragujevac, Kragujevac, Serbia;Department of Physiology, School of Medicine, University of Louisville, Louisville, KY, United States;

关键词: gut microbiota;    lipopolysaccharide;    matrix metalloproteinases;    Laser Doppler flowmetry;    periodontal disease;    Lactobacillus rhamnosus;   

DOI  :  10.3389/fphys.2021.625780

来源: DOAJ

【 摘 要 】

The gut microbiome has a very important role in human health and its influence on the development of numerous diseases is well known. In this study, we investigated the effect of high fat diet (HFD) on the onset of dysbiosis, gingival blood flow decreases, and the periodontal matrix remodeling. We established a dysbiosis model (HFD group) and probiotic model by Lactobacillus rhamnosus GG (LGG) treatment for 12weeks. Fecal samples were collected 24h before mice sacrificing, while short chain fatty acids (SCFA) analysis, DNA extraction, and sequencing for metagenomic analysis were performed afterwards. After sacrificing the animals, we collected periodontal tissues and conducted comprehensive morphological and genetic analyses. While HFD reduced Bacteroidetes, SCFA, and gingival blood flow, this type of diet increased Firmicutes, lipopolysaccharide (LPS) binding protein, TLR4, pro-inflammatory cytokines (TNF-α, IL-1β, and IL-6), matrix metalloproteinases (MMP-2 and MMP-9) expression, and also altered markers of bone resorption (OPG and RANKL). However, LGG treatment mitigated these effects. Thus, it was observed that HFD increased molecular remodeling via inflammation, matrix degradation, and functional remodeling and consequently cause reduced gingival blood flow. All of these changes may lead to the alveolar bone loss and the development of periodontal disease.

【 授权许可】

Unknown