Altering endoplasmic reticulum stress in a model of blast-induced traumatic brain injury controls cellular fate and ameliorates neuropsychiatric symptoms

Frontiers in Cellular Neuroscience

Rae Reiko Matsumoto¹  Matthew James Robson²  Ryan Coddington Turner³  Aric Flint Logsdon³  Brandon Peter Lucke-Wold³  Zachary James Naser³  Charles Lee Rosen³  Kelly Elizabeth Smith³  Jason Delwyn Huber³ 

[1] Touro University California College of Pharmacy;Vanderbilt University School of Medicine;West Virginia University Health Sciences Center;

关键词: Apoptosis;    Blood-Brain Barrier;    Endoplasmic Reticulum Stress;    Prefrontal Cortex;    Blast-Induced Traumatic Brain Injury;    Salubrinal;   

DOI  :  10.3389/fncel.2014.00421

来源: DOAJ

【 摘 要 】

Neuronal injury following blast-induced traumatic brain injury (bTBI) increases the risk for neuropsychiatric disorders, yet the pathophysiology remains poorly understood. Blood-brain-barrier (BBB) disruption, endoplasmic reticulum (ER) stress, and apoptosis have all been implicated in bTBI. Microvessel compromise is a primary effect of bTBI and is postulated to cause subcellular secondary effects such as ER stress. What remains unclear is how these secondary effects progress to personality disorders in humans exposed to head trauma. To investigate this we exposed male rats to a clinically relevant bTBI model we have recently developed. The study examined initial BBB disruption using Evan’s blue, ER stress mechanisms, apoptosis and impulsive-like behavior measured with elevated plus maze (EPM). Large BBB openings were observed immediately following bTBI, and persisted for at least 6 h. Data showed increased mRNA abundance of stress response genes at 3 h, with subsequent increases in the ER stress markers C/EBP homologous protein (CHOP) and growth arrest and DNA damage-inducible protein 34 (GADD34) at 24 h. Caspase-12 and Caspase-3 were both cleaved at 24 h following bTBI. The ER stress inhibitor, salubrinal (SAL), was administered (1mg/kg i.p.) to investigate its’ effects on neuronal injury and impulsive-like behavior associated with bTBI. SAL reduced CHOP protein expression, and diminished Caspase-3 cleavage, suggesting apoptosis attenuation. Interestingly, SAL also ameliorated impulsive-like behavior indicative of head trauma. These results suggest SAL plays a role in apoptosis regulation and the pathology of chronic disease. These observations provide evidence that bTBI involves ER stress and that the UPR is a promising molecular target for the attenuation of neuronal injury.

【 授权许可】

Unknown