Nanoplasmonic Structure of a Polycarbonate Substrate Integrated with Parallel Microchannels for Label-Free Multiplex Detection

Polymers

Hidetoshi Tahara¹  Te-Wei Chiu²  Yi-Jung Lu³  Horn-Jiunn Sheen⁴  Wen-Chang Kuo⁴  Han-Yun Hsieh⁴  Pei-Kuen Wei⁵  Yu-Jui Fan⁶ 

[1] Department of Cellular and Molecular Biology, Graduate School of Biomedical Sciences, Hiroshima University, Hiroshima 734-8553, Japan;Department of Materials and Mineral Resources Engineering, National Taipei University of Technology, Taipei 10608, Taiwan;Division of Family and Operative Dentistry, Department of Dentistry, Taipei Medical University Hospital, Taipei 11031, Taiwan;Institute of Applied Mechanics, National Taiwan University, Taipei 10617, Taiwan;Research Center for Applied Sciences, Academia Sinica, Nankang, Taipei 11529, Taiwan;School of Biomedical Engineering, Taipei Medical University, Taipei 11031, Taiwan;

关键词: surface plasmon resonance (SPR);    biosensor;    nanoimprinting;    multiplex detection;   

DOI  :  10.3390/polym13193294

来源: DOAJ

【 摘 要 】

In this study, a multiplex detection system was proposed by integrating a localized surface plasmon resonance (LSPR) sensing array and parallel microfluidic channels. The LSPR sensing array was fabricated by nanoimprinting and gold sputter on a polycarbonate (PC) substrate. The polydimethylsiloxane (PDMS) microfluidic channels and PC LSPR sensing array were bound together through (3-aminopropyl)triethoxysilane (APTES) surface treatment and oxygen plasma treatment. The resonant spectrum of the LSPR sensing device was obtained by broadband white-light illumination and polarized wavelength measurements with a spectrometer. The sensitivity of the LSPR sensing device was measured using various ratios of glycerol to water solutions with different refractive indices. Multiplex detection was demonstrated using human immunoglobulin G (IgG), IgA, and IgM. The anti-IgG, anti-IgA, and anti-IgM were separately modified in each sensing region. Various concentrations of human IgG, IgA, and IgM were prepared to prove the concept that the parallel sensing device can be used to detect different targets.

【 授权许可】

Unknown