Surface Plasmon Resonance for Cell-Based Clinical Diagnosis

Sensors

Yuhki Yanase¹  Takaaki Hiragun¹  Kaori Ishii¹  Tomoko Kawaguchi¹  Tetsuji Yanase¹  Mikio Kawai¹  Kenji Sakamoto² 

[1] Department of Dermatology, Division of Molecular Medical Science, Graduate School of Biomedical Science, Hiroshima University, 1-2-3, Kasumi, Minami-ku, Hiroshima 734-8551, Japan; E-Mails:;Center for Microelectronic systems, Kyushu Institute of Technology, 680-4, Kawazu, Iizuka, Fukuoka, 820-8502, Japan; E-Mail:

关键词: surface plasmon resonance (SPR);    SPR imaging (SPRI);    biosensor;    clinical diagnosis;    diagnosis of allergy;    diagnosis of cancer;    optic fiber SPR;   

DOI  :  10.3390/s140304948

来源: mdpi

【 摘 要 】

Non-invasive real-time observations and the evaluation of living cell conditions and functions are increasingly demanded in life sciences. Surface plasmon resonance (SPR) sensors detect the refractive index (RI) changes on the surface of sensor chips in label-free and on a real-time basis. Using SPR sensors, we and other groups have developed techniques to evaluate living cells' reactions in response to stimuli without any labeling in a real-time manner. The SPR imaging (SPRI) system for living cells may visualize single cell reactions and has the potential to expand application of SPR cell sensing for clinical diagnosis, such as multi-array cell diagnostic systems and detection of malignant cells among normal cells in combination with rapid cell isolation techniques.

【 授权许可】

CC BY   
© 2014 by the authors; licensee MDPI, Basel, Switzerland.