Detection of carbapenem-resistance genes in bacteria isolated from wastewater in Ontario

FACETS

Cassandra Carter¹  Hana McLeod¹  Prithika Sritharan¹  Marie Wright¹  Alex Wong²  Sandeep Tamber³  Burton W. Blais⁴  Ashley L. Cooper⁴  Catherine D. Carrillo⁵ 

[1] Applied Science and Environmental Technology, Algonquin College, Ottawa, ON K2G 1V8, Canada;Department of Biology, Carleton University, Ottawa, ON K1S 5B6, Canada;Microbiology Research Division, Bureau of Microbial Hazards, Health Canada, Ottawa, ON K1A 0K9, Canada;Research and Development, Canadian Food Inspection Agency, Ottawa, ON K1A 0Y9, Canada Department of Biology, Carleton University, Ottawa, ON K1S 5B6, Canada;Research and Development, Canadian Food Inspection Agency, Ottawa, ON K1A 0Y9, Canada;

关键词: carbapenem resistance;    whole-genome sequencing;    wastewater;    pcr;    β-lactamase;   

DOI  :  10.1139/facets-2020-0101

来源: DOAJ

【 摘 要 】

Bacterial carbapenem resistance is a major public health concern since these antimicrobials are often the last resort to treat serious human infections. To evaluate methodologies for detection of carbapenem resistance, carbapenem-tolerant bacteria were isolated from wastewater treatment plants in Toronto, Ottawa, and Arnprior, Ontario. A total of 135 carbapenem-tolerant bacteria were recovered. Polymerase chain reaction (PCR) indicated the presence of carbapenem hydrolysing enzymes KPC (n = 10), GES (n = 5), VIM (n = 7), and IMP (n = 1), and β-lactamases TEM (n = 7), PER (n = 1), and OXA-variants (n = 16). A subset of 46 isolates were sequenced and analysed using ResFinder and CARD-RGI. Both programs detected carbapenem resistance genes in 35 sequenced isolates and antimicrobial resistance genes (ARGs) conferring resistance to multiple class of other antibiotics. Where β-lactamase resistance genes were not initially identified, lowering the thresholds for ARG detection enabled identification of closely related β-lactamases. However, no known carbapenem resistance genes were found in seven sequenced Pseudomonas spp. isolates. Also of note was a multi-drug-resistant Klebsiella pneumoniae isolate from Ottawa, which harboured resistance to seven antimicrobial classes including β-lactams. These results highlight the diversity of genes encoding carbapenem resistance in Ontario and the utility of whole genome sequencing over PCR for ARG detection where resistance may result from an assortment of genes.

【 授权许可】

Unknown