Large-scale analysis of putative plasmids in clinical multidrug-resistant Escherichia coli isolates from Vietnamese patients

Frontiers in Microbiology

Microbiology

Juliette Hayer¹  Anne-Laure Bañuls¹  Dong Van Quyen²  Thi Thu Hang Le³  Son Thai Nguyen³  Tam Thi Thanh Tran³  Kieu-Oanh Thi Nguyen³  Quang Huy Nguyen⁴ 

[1] LMI DRISA, IRD-USTH, Hanoi, Vietnam;UMR MIVEGEC, University of Montpellier-IRD-CNRS, Montpellier, France;University of Science and Technology of Hanoi, Vietnam Academy of Science and Technology, Hanoi, Vietnam;Institute of Biotechnology, Vietnam Academy of Science and Technology, Hanoi, Vietnam;University of Science and Technology of Hanoi, Vietnam Academy of Science and Technology, Hanoi, Vietnam;LMI DRISA, IRD-USTH, Hanoi, Vietnam;University of Science and Technology of Hanoi, Vietnam Academy of Science and Technology, Hanoi, Vietnam;LMI DRISA, IRD-USTH, Hanoi, Vietnam;Institute of Biotechnology, Vietnam Academy of Science and Technology, Hanoi, Vietnam;

关键词: Escherichia coli;    putative plasmid;    carbapenem resistance;    horizontal gene transfer;    whole-genome sequencing;   

DOI  :  10.3389/fmicb.2023.1094119

 received in 2022-11-09, accepted in 2023-05-09,  发布年份 2023

来源: Frontiers

【 摘 要 】

IntroductionIn the past decades, extended-spectrum beta-lactamase (ESBL)-producing and carbapenem-resistant (CR) Escherichia coli isolates have been detected in Vietnamese hospitals. The transfer of antimicrobial resistance (AMR) genes carried on plasmids is mainly responsible for the emergence of multidrug-resistant E. coli strains and the spread of AMR genes through horizontal gene transfer. Therefore, it is important to thoroughly study the characteristics of AMR gene-harboring plasmids in clinical multidrug-resistant bacterial isolates.MethodsThe profiles of plasmid assemblies were determined by analyzing previously published whole-genome sequencing data of 751 multidrug-resistant E. coli isolates from Vietnamese hospitals in order to identify the risk of AMR gene horizontal transfer and dissemination.ResultsThe number of putative plasmids in isolates was independent of the sequencing coverage. These putative plasmids originated from various bacterial species, but mostly from the Escherichia genus, particularly E. coli species. Many different AMR genes were detected in plasmid contigs of the studied isolates, and their number was higher in CR isolates than in ESBL-producing isolates. Similarly, the blaKPC-2, blaNDM-5, blaOXA-1, blaOXA-48, and blaOXA-181 β-lactamase genes, associated with resistance to carbapenems, were more frequent in CR strains. Sequence similarity network and genome annotation analyses revealed high conservation of the β-lactamase gene clusters in plasmid contigs that carried the same AMR genes.DiscussionOur study provides evidence of horizontal gene transfer in multidrug-resistant E. coli isolates via conjugative plasmids, thus rapidly accelerating the emergence of resistant bacteria. Besides reducing antibiotic misuse, prevention of plasmid transmission also is essential to limit antibiotic resistance.

【 授权许可】

Unknown   
Copyright © 2023 Nguyen, Le, Nguyen, Nguyen, Dong, Hayer, Bañuls and Tran.