KIT Mutations Correlate with Higher Galectin Levels and Brain Metastasis in Breast and Non-Small Cell Lung Cancer

Cancers

Joseph A. Ewing¹  Bailey B. Blair²  Avery T. Funkhouser²  Alexander M. Strigenz²  Anna V. Blenda²  Andrew P. Miller²  Jonah C. Shealy²  Christopher R. Funk³  Julie C. Martin⁴  William J. Edenfield⁴ 

[1] Data Support Core, Prisma Health, Greenville, SC 29605, USA;Department of Biomedical Sciences, University of South Carolina School of Medicine Greenville, Greenville, SC 29605, USA;Department of Hematology and Medical Oncology, Emory University School of Medicine, Atlanta, GA 30322, USA;Prisma Health Cancer Institute, Prisma Health, Greenville, SC 29605, USA;

关键词: galectin;    KIT gene;    mutation;    c-Kit;    CD117;    breast cancer;   

DOI  :  10.3390/cancers14112781

来源: DOAJ

【 摘 要 】

To investigate a potential role for galectins as biomarkers that enable diagnosis or prognostication of breast or non-small cell lung cancer, the serum levels of galectins -1, -3, -7, -8, and -9 of cancer patients determined by ELISA assays were compared to the mutation status of 50 known cancer-critical genes, which were determined using multiplex PCR in tumors of the same patients. Mutations in the KIT proto-oncogene, which codes for the c-Kit protein, a receptor tyrosine kinase, correlated with higher levels of galectins -1, -3, -8, and -9 in breast cancer patients and galectin-1 in non-small cell lung cancer patients. Mutations in the KIT gene were more likely found in brain metastases from both of these primary cancers. The most common KIT mutation in our panel was p.M541L, a missense mutation in the transmembrane domain of the c-Kit protein. These results demonstrate an association between KIT oncogenic signaling and elevated serum galectins in patients with metastatic disease. Changes in protein trafficking and the glycocalyx composition of cancer cells may explain the observed alterations in galectin expression. This study can be useful for the targeted selection of receptor tyrosine kinase and galectin inhibitor anti-cancer treatments.

【 授权许可】

Unknown