Bactericidal and Biocompatible Properties of Plasma Chemical Oxidized Titanium (TiOB®) with Antimicrobial Surface Functionalization

Materials

André Guellmar¹  Bernd Sigusch¹  Tobias Lesser¹  Andrea Voelpel¹  Stefan Kranz¹  Silke Tonndorf-Martini¹  Wolfgang Pfister²  Michael Diefenbeck³  Juergen Schmidt⁴  Christian Schrader⁴  Ulrich Finger⁵  Mathilde Faucon⁵ 

[1] Department of Conservative Dentistry and Periodontology, University Hospital Jena, An der Alten Post 4, 07743 Jena, Germany;Department of Microbiology, University Hospital Jena, Erlanger Allee 101, 07747 Jena, Germany;Department of Trauma-, Hand- and Reconstructive Surgery, University Hospital Jena, Erlanger Allee 101, 07747 Jena, Germany;Innovent Technology Development, Prüssingstraße 27 B, 07745 Jena, Germany;Königsee Implantate GmbH, OT-Aschau—Am Sand 4, 07426 Allendorf, Germany;

关键词: titanium implants;    dental implants;    antibacterial coating;    gentamicin;    silver;    zinc;    cytotoxicity;    MC3T3-E1;    Staphylococcus aureus;    plasma chemical oxidation;   

DOI  :  10.3390/ma12060866

来源: DOAJ

【 摘 要 】

Coating of plasma chemical oxidized titanium (TiOB®) with gentamicin-tannic acid (TiOB® gta) has proven to be efficient in preventing bacterial colonization of implants. However, in times of increasing antibiotic resistance, the development of alternative antimicrobial functionalization strategies is of major interest. Therefore, the aim of the present study is to evaluate the antibacterial and biocompatible properties of TiOB® functionalized with silver nanoparticles (TiOB® SiOx Ag) and ionic zinc (TiOB® Zn). Antibacterial efficiency was determined by agar diffusion and proliferation test on Staphylocuccus aureus. Cytocompatibility was analyzed by direct cultivation of MC3T3-E1 cells on top of the functionalized surfaces for 2 and 4 d. All functionalized surfaces showed significant bactericidal effects expressed by extended lag phases (TiOB® gta for 5 h, TiOB® SiOx Ag for 8 h, TiOB® Zn for 10 h). While TiOB® gta (positive control) and TiOB® Zn remained bactericidal for 48 h, TiOB® SiOx Ag was active for only 4 h. After direct cultivation for 4 d, viable MC3T3-E1 cells were found on all surfaces tested with the highest biocompatibility recorded for TiOB® SiOx Ag. The present study revealed that functionalization of TiOB® with ionic zinc shows bactericidal properties that are comparable to those of a gentamicin-containing coating.

【 授权许可】

Unknown