Expanded genetic screening in Caenorhabditis elegans identifies new regulators and an inhibitory role for NAD+ in axon regeneration

eLife

Ngang Heok Tang¹  Zilu Wu¹  Ming Zhu¹  Yishi Jin¹  Matthew G Andrusiak¹  Kyung Won Kim¹  Andrew D Chisholm¹  Christopher A Piggott¹  Seungmee Park¹  Naina Kurup¹  Salvatore J Cherra III¹ 

[1] Section of Neurobiology, Division of Biological Sciences, University of California, San Diego, La Jolla, United States;

关键词: NMNAT;    Kelch-domain protein;    axon reconnection/fusion;    membrane contact site (MCS);    phospholipid metabolic enzyme;    membrane transporter;   

DOI  :  10.7554/eLife.39756

来源: DOAJ

【 摘 要 】

The mechanisms underlying axon regeneration in mature neurons are relevant to the understanding of normal nervous system maintenance and for developing therapeutic strategies for injury. Here, we report novel pathways in axon regeneration, identified by extending our previous function-based screen using the C. elegans mechanosensory neuron axotomy model. We identify an unexpected role of the nicotinamide adenine dinucleotide (NAD+) synthesizing enzyme, NMAT-2/NMNAT, in axon regeneration. NMAT-2 inhibits axon regrowth via cell-autonomous and non-autonomous mechanisms. NMAT-2 enzymatic activity is required to repress regrowth. Further, we find differential requirements for proteins in membrane contact site, components and regulators of the extracellular matrix, membrane trafficking, microtubule and actin cytoskeleton, the conserved Kelch-domain protein IVNS-1, and the orphan transporter MFSD-6 in axon regrowth. Identification of these new pathways expands our understanding of the molecular basis of axonal injury response and regeneration.

【 授权许可】

Unknown