Biochemical, transcriptomic, gut microbiome responses and defense mechanisms of the earthworm Eisenia fetida to salt stress

Ecotoxicology and Environmental Safety

Xing Wang¹  Guangshen Shang²  Xuelian Yang³ 

[1] Beijing Key Laboratory of Biodiversity and Organic Farming, Beijing 100193, China;Organic Recycling Research Institute (Suzhou) of China Agricultural University, Suzhou 215128, China;College of Resources and Environmental Sciences, China Agricultural University, Beijing 100193, China;

关键词: Reproduction;    ROS;    DNA damage;    Soil invertebrate;    Ecotoxicity;   

DOI  :  

来源: DOAJ

【 摘 要 】

The accumulation of sodium chloride (NaCl) in soil is a worldwide problem with detrimental effects on the survival of soil animals. The effects of NaCl on earthworms remain unclear. Here, we show that the growth rate, cocoon production rate, annetocin precursor (ANN) mRNA level, and superoxide dismutase and catalase activities in earthworms were reduced under NaCl stress, whereas the mortality rate, reactive oxygen species (ROS) and malondialdehyde activity level increased. Histological damage to the earthworm body wall and intestine were observed under NaCl stress. NaCl stress increased DNA damage in the seminal vesicle and coelomocytes. The mRNA level of lumbrokinase, 1,3-beta-glucanse, coelomic cytolytic factor (CCF1), and alpha-amylase was significantly down-regulated, whereas that of earthworm excitatory peptides2 (EEP2) was up-regulated. From 16 S rRNA sequencing, the earthworm gut microbiota diversity decreased under NaCl stress. However, Verminephrobacter, Kluyvera, Lactobacillus, and Ochrobactrum increased under NaCl stress. These findings contribute to the risk assessment of the salt stress on soil organisms.

【 授权许可】

Unknown