Metabolomic Studies of Lipid Storage Disorders, with Special Reference to Niemann-Pick Type C Disease: A Critical Review with Future Perspectives

International Journal of Molecular Sciences

FrancesM. Platt¹  BenitaClaire Percival²  Miles Gibson²  Martin Grootveld²  PhilippeB. Wilson³ 

[1] Department of Pharmacology, University of Oxford, Mansfield Rd, Oxford OX1 3QT, UK;Leicester School of Pharmacy, De Montfort University, The Gateway, Leicester LE1 9BH, UK;School of Animal, Rural and Environmental Sciences, Brackenhurst Campus, Nottingham Trent University, Southwell NG25 0QF, UK;

关键词: metabolomics;    lysosomal storage disorders;    Niemann-Pick Type C Disease;    lipidoses;    nuclear magnetic resonance (NMR) analysis;    liquid chromatography-mass spectrometric (LC-MS) analysis;   

DOI  :  10.3390/ijms21072533

来源: DOAJ

【 摘 要 】

Lysosomal storage disorders (LSDs) are predominantly very rare recessive autosomal neurodegenerative diseases.Sphingolipidoses, a sub-group of LSDs, result from defects in lysosomal enzymes involved in sphingolipid catabolism, and feature disrupted storage systems which trigger complex pathogenic cascades with other organelles collaterally affected. This process leads to cell dysfunction and death, particularly in the central nervous system. One valuable approach to gaining insights into the global impact of lysosomal dysfunction is through metabolomics, which represents a discovery tool for investigating disease-induced modifications in the patterns of large numbers of simultaneously-analysed metabolites, which also features the identification of biomarkers Here, the scope and applications of metabolomics strategies to the investigation of sphingolipidoses is explored in order to facilitate our understanding of the biomolecular basis of these conditions. This review therefore surveys the benefits of applying ’state-of-the-art’ metabolomics strategies, both univariate and multivariate, to sphingolipidoses, particularly Niemann-Pick type C disease. Relevant limitations of these techniques are also discussed, along with the latest advances and developments. We conclude that metabolomics strategies are highly valuable, distinctive bioanalytical techniques for probing LSDs, most especially for the detection and validation of potential biomarkers. They also show much promise for monitoring disease progression and the evaluation of therapeutic strategies and targets.

【 授权许可】

Unknown