Potential targets of TMEM176A in the growth of glioblastoma cells

OncoTargets and Therapy

关键词: TMEM176A;    cell cycle;    cell apoptosis;    ERK1/2;    glioblastomas;   

DOI  :  

来源: DOAJ

【 摘 要 】

Zhiguo Liu,1 Haixia An,2 Peng Song,3 Dejing Wang,4 Shichun Li,5 Kai Chen,6 Qi Pang71Department of Neurosurgery, People’s Hospital of Zhangqiu, Shandong Provincial Hospital Affiliated to Shandong University, Zhangqiu, Jinan, Shandong 250200, People’s Republic of China; 2Department of Oncology, Jinan Zhangqiu Hospital of Traditional Chinese Medicine, Zhangqiu, Jinan, Shandong 250200, People’s Republic of China; 3Department of Orthopedics, People’s Hospital of Zhangqiu, Zhangqiu, Jinan, Shandong 250200, People’s Republic of China; 4Department of Stomatology, People’s Hospital of Zhangqiu, Zhangqiu, Jinan, Shandong 250200, People’s Republic of China; 5Department of Doppler Ultrasonic, People’s Hospital of Zhangqiu, Zhangqiu, Jinan, Shandong 250200, People’s Republic of China; 6Department of Neurology, The Fourth People’s Hospital of Jinan, Tianqiao, Jinan, Shandong 250200, People’s Republic of China; 7Department of Neurosurgery, Shandong Provincial Hospital Affiliated to Shandong University, Huaiyin, Jinan, Shandong 250200, People’s Republic of ChinaBackground: Human transmembrane protein 176A (TMEM176A) is upregulated in several tumors. Growing evidence has suggested the high clinical value of TMEM176A as a biomarker for early tumor diagnosis. However, less is known about the function of TMEM176A in glioblastomas (GBMs).Methods: In this study, we systematically analyzed the effect of TMEM176A knockdown and overexpression in GBM cells (U87, T98G and A172) on cell proliferation, cell cycle and cell apoptosis.Results: Our results indicated that TMEM176A acted as a tumor-promoting factor in GBM cells. Moreover, a specific ERK1/2 inhibitor, U0126, suppressed the function of TMEM176A in GBM cells. Therefore, we proposed that TMEM176A may be involved in a pathway including ERK1/2 in the regulation of the cell cycle. Moreover, we also found that TMEM176A affected the expression of Bcl2 and played a central role in apoptosis of GBM cells.Conclusion: Taken together, our results not only elucidated the multiple functions of TMEM176A in GBM cells but also provided a deep insight into the potential targets of TMEM176A in the growth of GBM cells.Keywords: TMEM176A, cell cycle, cell apoptosis, ERK1/2, glioblastomas

【 授权许可】

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