Preparation and Integration of a Multi-Wavelength LED Matrix for Testing Light Cell Interaction in a Novel Lens Less Optical Microscope

Proceedings

Rudolf Heer¹  Jörg Schotter¹  Silvana Geleff²  Sigurd Krieger²  Wenze Wu³  Gregor Scholz³  Hutomo Suryo Wasisto³ 

[1] AIT Austrian Institute of Technology GmbH, Giefinggasse 4, 1210 Vienna, Austria;Department of Pathology, Medical University of Vienna, Währinger Gürtel 18-20, 1090 Vienna, Austria;Institute of Semiconductor Technology (IHT) and Laboratory for Emerging Nanometrology (LENA), Technische Universität Braunschweig, 38106 Braunschweig, Germany;

关键词: imaging of cells;    cell light interaction;    microfluidic;    lens less imaging;   

DOI  :  10.3390/proceedings2131074

来源: DOAJ

【 摘 要 】

In this work we studied the influence of light emitting diode (LED) generated light on living cells which were cultivated in common cell culture microtiter plates. In detail we investigated signaling side effects including apoptosis by the use of a cell permeable peroxide activatable fluorescent dye (5,6-Chloromethyl-2′,7′-dichlorodihydrofluorescein diacetate). A high level production of peroxides in UV and blue light exposed cells was measured while the light of longer wavelengths caused only minor effects on the cells.

【 授权许可】

Unknown