Preparation and Integration of a Multi-Wavelength LED Matrix for Testing Light Cell Interaction in a Novel Lens Less Optical Microscope

Proceedings

Heer, Rudolf¹  Schotter, Jörg²  Krieger, Sigurd³  Geleff, Silvana⁴  Wu, Wenze⁵ 

[1] AIT Austrian Institute of Technology GmbH, Giefinggasse 4, 1210 Vienna, Austria;Author to whom correspondence should be addressed.;Department of Pathology, Medical University of Vienna, Währinger Gürtel 18-20, 1090 Vienna, Austria;Institute of Semiconductor Technology (IHT) and Laboratory for Emerging Nanometrology (LENA), Technische Universität Braunschweig, 38106 Braunschweig, Germany;Presented at the Eurosensors 2018 Conference, Graz, Austria, 9–12 September 2018

关键词: imaging of cells;    cell light interaction;    microfluidic;    lens less imaging;   

DOI  :  10.3390/proceedings2131074

学科分类：社会科学、人文和艺术（综合）

来源: mdpi

【 摘 要 】

In this work we studied the influence of light emitting diode (LED) generated light on living cells which were cultivated in common cell culture microtiter plates. In detail we investigated signaling side effects including apoptosis by the use of a cell permeable peroxide activatable fluorescent dye (5,6-Chloromethyl-2′,7′-dichlorodihydrofluorescein diacetate). A high level production of peroxides in UV and blue light exposed cells was measured while the light of longer wavelengths caused only minor effects on the cells.

【 授权许可】

CC BY