Characterization of Inulin-Type Fructan from Platycodon grandiflorus and Study on Its Prebiotic and Immunomodulating Activity

Molecules

Xue-Qin Ni¹  Bin Feng²  Frode Rise³  BeritSmestad Paulsen⁴  Ren-Yong Jia⁵  Zheng-Li Chen⁵  Xu Song⁶  Yuan-Feng Zou⁶  Yu-Ping Fu⁶  Chao Huang⁶  Mei-Ling Chen⁶  Zhong-Qiong Yin⁶  De-Jiang Pang⁶  Li-Xia Li⁶  Bing-Zhao Zhang⁷  Yu-Long Chen⁸ 

[1] Animal Microecology Institute, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China;Animal Nutrition Institute, Sichuan Agricultural University, Chengdu 611130, China;Department of Chemistry, University of Oslo, P.O. Box 1033, Blindern, 0315 Oslo, Norway;Department of Pharmaceutical Chemistry, School of Pharmacy, University of Oslo, P.O. Box 1068, Blindern, 0316 Oslo, Norway;Key Laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China;Natural Medicine Research Center, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China;Shenzhen Institutes of Advanced Technology, Chinese Academy of Science, Shenzhen 518055, China;Sichuan Academy of Forestry, Chengdu 610081, China;

关键词: Platycodon grandiflorus;    inulin-type fructan;    prebiotic activity;    immunomodulation;   

DOI  :  10.3390/molecules24071199

来源: DOAJ

【 摘 要 】

Platycodon grandiflorus is a plant widely used in traditional Chinese medicine, of which polysaccharides are reported to be the main components responsible for its bio-functions. In this work, the inulin-type fructan (PGF) was obtained by DEAE anion exchange chromatography from the water extracted from P. grandifloras. Characterization was performed with methanolysis, methylation, and NMR and the results showed that PGF is a β-(2-1) linked fructan, with terminal glucose and with a degree of polymerization of 2–10. In order to study its biofunctions, the prebiotic and immunomodulation properties were assayed. We found that PGF exhibited good prebiotic activity, as shown by a promotion on six strains of lactobacillus proliferation. Additionally, the PGF also displayed direct immunomodulation on intestinal epithelial cells and stimulated the expressions of anti-inflammatory factors. These results indicated that the inulin from P. grandiflorus is a potential natural source of prebiotics as well as a potential intestinal immunomodulator, which will be valuable for further studies and new applications.

【 授权许可】

Unknown