Genome-Wide Development of Polymorphic Microsatellite Markers and Association Analysis of Major Agronomic Traits in Core Germplasm Resources of Tartary Buckwheat

Frontiers in Plant Science

Longlong Liu¹  Wei Du²  Donghang Wang²  Xinfang Wang²  Zhaoxia Sun³  Hongying Li³  Siyu Hou³  Yang Yang³  Yuanhuai Han³  Xuemei Ren³ 

[1]Center for Agricultural Genetic Resources Research, Shanxi Agricultural University, Taiyuan, China

[2]College of Agriculture, Institute of Agricultural Bioengineering, Shanxi Agricultural University, Taigu, China

[3]Shanxi Key Laboratory of Minor Crop Germplasm Innovation and Molecular Breeding, Taiyuan, China

关键词: Tartary buckwheat;    genome-wide SSR marker development;    association study;    core germplasm;    agronomic traits;   

DOI  :  10.3389/fpls.2022.819008

来源: DOAJ

【 摘 要 】

Tartary buckwheat (TB; Fagopyrum tataricum Gaertn.) is an important multigrain crop and medicinal plant, but functional genomics and molecular breeding research in this species have been lacking for quite some time. Here, genome-wide screening was performed to develop simple sequence repeat (SSR) markers associated with six major agronomic traits and the rutin contents of 97 core germplasm resources. A total of 40,901 SSR loci were identified; they were uniformly distributed throughout the TB genome, with a mean distance of 11 kb between loci. Based on these loci, 8,089 pairs of SSR primers were designed, and 101 primer pairs for polymorphic SSR loci were used to genotype the 97 core germplasm resources. The polymorphic SSR loci showed high genetic variation in these core germplasm resources, with an average polymorphic information content (PIC) value of 0.48. In addition, multiple SSR markers, such as SXAU8002 [100-grain weight (HGW)] and SXAU8006 [stem diameter (SD)], were found to be associated with agronomic traits in the two environments. Finally, based on gene functional annotation and homology analysis, a candidate gene, FtPinG0007685500, that may affect the node number and SD of the main stem by participating in lignin synthesis was identified. This study reports the mining of genome-wide SSR loci and the development of markers in TB, which can be used for molecular characterization of the germplasm in its gene pool. In addition, the detected markers and candidate genes could be used for marker-assisted breeding and functional gene cloning in TB.

【 授权许可】

Unknown