Bioactivity comparison of extracts from various parts of common and tartary buckwheats: evaluation of the antioxidant- and angiotensin-converting enzyme inhibitory activities

Chemistry Central Journal

Yahsien Hsu¹  Shangheng Tsai¹  Hweiwen Deng¹  Hweiyan Tsai² 

[1]Department of Applied Chemistry, Chung Shan Medical University, Taichung, 402, Taiwan

[2]Clinical Laboratory, Chung Shan Medical University Hospital, Taichung, 402, Taiwan

关键词: Microplate fluometric assay;    ACE inhibition;    Common buckwheat;    Tartary buckwheat;   

Others  :  788098
DOI  :  10.1186/1752-153X-6-78

 received in 2012-06-21, accepted in 2012-06-28,  发布年份 2012

【 摘 要 】

Background

Buckwheat flour and buckwheat sprouts possess antioxidant properties, and previous studies have reported on buckwheat flour displaying an inhibitory activity for angiotensin-I converting enzyme (ACE). Information is lacking on the bioactivity of other parts of the buckwheat, such as the seed hulls and plant stalks. This study investigates the ACE inhibitory activity and antioxidant activity of various parts of 2 types of buckwheat, namely, common buckwheat (Fagopyrum esculentum Moench) and tartary buckwheat (Fagopyrum tataricum Gaertn).

Results

The extract of common hulls extracted using 50% (v/v)-ethanol solvent presented a remarkable inhibitory activity. The value of IC₅₀ is 30 μg ml^-1. The extracts of both common and tartary hulls extracted using 50% (v/v)-ethanol solvent demonstrated an antioxidant activity that is superior to that of other extracts.

Conclusion

This study determined that the ethanolic extract of the hulls of common buckwheat presented more favorable antioxidant and ACE inhibitory abilities. However, the correlation of antioxidant activity and ACE inhibitory activity for all 18 types of extracts is low. The ACE inhibitory activity could have been caused by a synergistic effect of flavonoids or from other unidentified components in the extracts. The ethanolic extract of common hulls demonstrated remarkable ACE inhibitory activity and is worthy of further animal study.

【 授权许可】

   
2012 Tsai et al.; licensee Chemistry Central Ltd.

【 图 表 】

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【 参考文献 】

• [1]Bonafaccia G, Marocchini M, Kreft I: Composition and technological properties of the flour and bran from common and tartary buckwheat. Food Chem 2003, 80:9-15.
• [2]Gawlik-Dziki U, Dziki D, Baraniak B, Lin R: The effect of simulated digestion in vitro on bioactivity of wheat bread with Tartary buckwheat flavones addition. LWT- Food Sci Technol 2009, 42:137-143.
• [3]Górecka D, Heś M, Szymandera-Buszka K, Dziedzic K: Contents of selected bioactive components in buckwheat groats. Acta Sci Polonorum, Technologia Alimentaria 2009, 8:75-83.
• [4]Holasova M, Fiedlerova V, Smrcinova H, Orsak M, Lachman J, Vavreinova S: Buckwheat–the source of antioxidant activity in functional foods. Food Res Int 2002, 35:207-211.
• [5]Jiang P, Burczynski F, Campbell C, Pierce G, Austria JA, Briggs CJ: Rutin and flavonoid contents in three buckwheat species Fagopyrum esculentum, F. tataricum, and F. homotropicum and their protective effects against lipid peroxidation. Food Res Int 2007, 40:356-364.
• [6]Kreft I, Fabjan N, Yasumoto K: Rutin content in buckwheat (Fagopyrum esculentum Moench) food materials and products. Food Chem 2006, 98:508-512.
• [7]Liu B, Zhu Y: Extraction of flavonoids from flavonoid-rich parts in tartary buckwheat and identification of the main flavonoids. J Food Eng 2007, 78:584-587.
• [8]Ma Y, Xiong YL, Zhai J, Zhu H, Dziubla T: Fractionation and evaluation of radical scavenging peptides from in vitro digests of buckwheat protein. Food Chem 2010, 118:582-588.
• [9]Sun T, Ho C-T: Antioxidant activities of buckwheat extracts. Food Chem 2005, 90:743-749.
• [10]Suzuki T, Honda Y, Funatsuki W, Nakatsuka K: Purification and characterization of flavonol 3-glucosidase, and its activity during ripening in tartary buckwheat seeds. Plant Sci 2002, 163:417-423.
• [11]Zheng GH, Sosulski FW, Tyler RT: Wet-milling, composition and functional properties of starch and protein isolated from buckwheat groats. Food Res Int 1997, 30:493-502.
• [12]Zieliński H, Michalska A, Piskuła MK, Kozłowska H: Antioxidants in thermally treated buckwheat groats. Mol Nutr Food Res 2006, 50:824-832.
• [13]Campbell CG: Buckwheat. Fagopyrum esculentum Moench. Promoting the conservation and use of underutilized and neglected crops. Rome, Italy: The International Plant Genetic Resources Institute (IPGRI); 1997:23-25.
• [14]Lacaille-Dubois MA, Franck U, Wagner H: Search for potential Angiotensin Converting Enzyme (ACE)-inhibitors from plants. Phytomedicine 2001, 8:47-52.
• [15]Tsutsumi Y, Shimada A, Miyano A, Nishida T, Mitsunaga T: In vitro screening of angiotensin I-converting enzyme inhibitors from Japanese cedar (Cryptomeria japonica). J Wood Sci 1998, 44:463-468.
• [16]Aoyagi Y: An angiotensin-I converting enzyme inhibitor from buckwheat (Fagopyrum esculentum Moench) flour. Phytochemistry 2006, 67:618-621.
• [17]Li CH, Matsui T, Matsumoto K, Yamasaki R, Kawasaki T: Latent production of angiotensin I-converting enzyme inhibitors from buckwheat protein. J Pept Sci 2002, 8:267-274.
• [18]Ma MS, In YB, Hyeon GL, Yang CB: Purification and identification of angiotensin I-converting enzyme inhibitory peptide from buckwheat (Fagopyrum esculentum Moench). Food Chem 2006, 96:36-42.
• [19]Pihlanto-Leppala A, Rokka T, Korhonen H: Angiotensin I Converting Enzyme Inhibitory Peptides Derived from Bovine Milk Proteins. Int Dairy J 1998, 8:325-331.
• [20]Dietrych-Szostak D, Oleszek W: Effect of Processing on the Flavonoid Content in Buckwheat (Fagopyrum esculentum Moench) Grain. J Agric Food Chem 1999, 47:4384-4387.
• [21]Schwager SL, Carmona AK, Sturrock ED: A high-throughput fluorimetric assay for angiotensin I-converting enzyme. Nat Protoc 2006, 1:1961-1964.
• [22]Roth M: Fluorescence reaction for amino acids. Anal Chem 1971, 43:880-882.
• [23]Benzie IFF, Strain JJ: The Ferric Reducing Ability of Plasma (FRAP) as a Measure of "Antioxidant Power": The FRAP Assay. Anal Biochem 1996, 239:70-76.