ACE inhibition and antioxidant activity of different part of Channa striata prepared by various cooking method

2nd International Symposium on Marine and Fisheries Research

地球科学;农业科学

Chasanah, E.^1 ; Budiari, S.^2 ; Thenawijaya, M.^2 ; Palupi, N.S.^2

Research Center for Marine and Fisheries Product Processing and Biotechnology, Jl. KS Tubun Petamburan 6, Jakarta, Indonesia^1

Food Science Program, IPB, Darmaga Bogor, Indonesia^2

关键词: Ace activities;    ACE inhibition;    Angiotensin-converting enzyme;    Anti-oxidant activities;    Cooking methods;    Dpph methods;    Heat processing;    L-leucine;   

Others  :  https://iopscience.iop.org/article/10.1088/1755-1315/139/1/012044/pdf
DOI  :  10.1088/1755-1315/139/1/012044

学科分类：农业科学（综合）

来源: IOP

【 摘 要 】

Channa striata (snakehead) extract has been known possessing positive activity, one of which is the ability to inhibit Angiotensin Converting Enzyme (ACE) activity in vitro. Aims of this study were to determine the effect of cooking and parts of C. striata, i.e. meat/fillet, gonad, skin, gill against the ACE inhibition activity and antioxidant activity in vitro. Heat processing methods used were direct boiling and indirect boiling and steamed at 100 C for 10 min. ACE inhibition activity was analyzed using hippuryl-L-histidyl-L-leucine (HHL) as substrate and antioxidant activity was analyzed using DPPH method. The result shows that the higher the concentration of the extract (5 %, 20 %, 35 % and 50 %), the higher the antioxidant activity. The highest antioxidant activity was shown by gonad followed by meat extract, skin, and gill. Cooking treatment affected antioxidant activity, being the detrimental treatment were steam and direct boiling. The egg/gonad of C. striata showed the highest capability to inhibit ACE activity followed by meat/fillet, gill and skin. In concentration of 10 mg, extract of C. striata gonad was comparable to captopril, a commercial hypertension drug. While uncooked fillet showed the highest ACE inhibition activity followed by indirect boiling, direct boiling and steaming.