Seeing beyond the tip of the iceberg: A deep analysis of the venome of the Brazilian Rattlesnake, Crotalus durissus terrificus

EuPA Open Proteomics

Paulo C. Carvalho¹  Livia Goto²  Magno Junqueira²  Gilberto B. Domont²  Gabriel D.T. Araujo²  Fábio C.S. Nogueira²  Rafael D. Melani² 

[1] Laboratory for Proteomics and Protein Engineering, Carlos Chagas Institute, Fiocruz, Paraná, Brazil;Proteomics Unit, Rio de Janeiro Proteomics Network, Departamento de Bioquímica. Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil;

关键词: Crotalus durissus terrificus venom;    In-solution digestion;    IEF;    CPLL;    Bottom-up approaches;    Shotgun;   

DOI  :  10.1016/j.euprot.2015.05.006

来源: DOAJ

【 摘 要 】

The complete characterization of the snake venom protein components is a requirement for a systems-wide understanding of their biological context. In this work, we provide a deep proteomic characterization of Crotalus durissus terrificus venom using different bottom-up approaches. We identified more than five times more protein families than the sum of all identifications previously reported. For the first time in this sub-species, we report the identification of three new toxin families: CRISP, phospholipase-B, and SVVEGF. This work also describes proteins involved in regulation of toxin synthesis and processing that are present in venom.

【 授权许可】

Unknown