Cryopreservation Differentially Alters the Proteome of Epididymal and Ejaculated Pig Spermatozoa

International Journal of Molecular Sciences

Heriberto Rodriguez-Martinez¹  Isabel Barranco²  Cristina Perez-Patiño²  EmilioA. Martinez²  Inmaculada Parrilla²  Lorena Padilla²  Jordi Roca²  Junwei Li² 

[1] Department of Clinical and Experimental Medicine, Faculty of Medicine and Health Sciences, Linköping University, SE-58185 Linköping, Sweden;Department of Medicine and Animal Surgery, Veterinary Science, University of Murcia, 30100 Murcia, Spain;

关键词: epididymis;    ejaculate;    spermatozoa;    cryopreservation;    proteomics;    porcine;   

DOI  :  10.3390/ijms20071791

来源: DOAJ

【 摘 要 】

Cryopreservation induces differential remodeling of the proteome in mammalian spermatozoa. How these proteome changes relate to the loss of sperm function during cryopreservation remains unsolved. The present study aimed to clarify this issue evaluating differential changes in the proteome of fresh and frozen-thawed pig spermatozoa retrieved from the cauda epididymis and the ejaculate of the same boars, with clear differences in cryotolerance. Spermatozoa were collected from 10 healthy, sexually mature, and fertile boars, and cryopreserved using a standard 0.5 mL-straw protocol. Total and progressive motility, viability, and mitochondria membrane potential were higher and membrane fluidity and reactive oxygen species generation lower in frozen-thawed (FT) epididymal than ejaculated spermatozoa. Quantitative proteomics of fresh and FT spermatozoa were analyzed using a LC-ESI-MS/MS-based Sequential Window Acquisition of All Theoretical Spectra approach. Cryopreservation quantitatively altered more proteins in ejaculated than cauda epididymal spermatozoa. Differential protein–protein networks highlighted a set of proteins quantitatively altered in ejaculated spermatozoa, directly involved in mitochondrial functionality which would explain why ejaculated spermatozoa deteriorate during cryopreservation.

【 授权许可】

Unknown