Docosahexaenoic Acid Inhibits PTP1B Phosphatase and the Viability of MCF-7 Breast Cancer Cells

Nutrients

Magdalena Gorska-Ponikowska¹  Michal Wozniak¹  Tomasz Kostrzewa¹  Alicja Kuban-Jankowska¹  Jack Tuszynski²  KamleshKumar Sahu³ 

[1] Department of Medical Chemistry, Medical University of Gdansk, 1 Debinki St., 80-211 Gdansk, Poland;Department of Oncology, University of Alberta, Edmonton, AB T6G 1Z2, Canada;Li Ka Shing Applied Virology Institute, Department of Medical Microbiology and Immunology 6-020 Katz Group Centre, University of Alberta, Edmonton, AB T6G 2E1, Canada;

关键词: breast cancer;    docosahexaenoic acid;    omega-3 acids;    protein tyrosine phosphatase ptp1b;   

DOI  :  10.3390/nu11112554

来源: DOAJ

【 摘 要 】

Background: Docosahexaenoic acid (DHA) is an essential polyunsaturated fatty acid compound present in deep water fishes and dietary supplements, with a wide spectrum of potential health benefits, ranging from neurological to anti-inflammatory. Methods: Due to the fact that DHA is considered a breast cancer risk reducer, we examined the impact of DHA on MCF-7 breast cancer cells’ viability and its inhibitory properties on protein tyrosine phosphatase 1B (PTP1B), a pro-oncogenic phosphatase. Results: We found that DHA is able to lower both the enzymatic activity of PTP1B phosphatase and the viability of MCF-7 breast cancer cells. We showed that unsaturated DHA possesses a significantly higher inhibitory activity toward PTP1B in comparison to similar fatty acids. We also performed a computational analysis of DHA binding to PTP1B and discovered that it is able to bind to an allosteric binding site. Conclusions: Utilizing both a recombinant enzyme and cellular models, we demonstrated that DHA can be considered a potential pharmacological agent for the prevention of breast cancer.

【 授权许可】

Unknown