Detection of Two Highly Diverse Peribunyaviruses in Mosquitoes from Palenque, Mexico

Viruses

Anne Kopp¹  Sandra Junglen¹  Alexandra Hübner¹  Christian Drosten¹  Daniel Hobelsberger²  ThomasR. Gillespie³  Alejandro Estrada⁴  Florian Zirkel⁵  Ingo Jordan⁶ 

[1] Berlin Institute of Health, Institute of Virology, Charité—Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, 10117 Berlin, Germany;Department of Biology, Universität Regensburg, 93053 Regensburg, Germany;Department of Environmental Sciences and Program in Population Biology, Ecology and Evolution, Emory University, Atlanta, GA 30322, USA;Estación de Biología Tropical Los Tuxtlas, Instituto de Biología, Universidad Nacional Autónoma de México, Mexico City 04513, Mexico;Institute of Virology, University of Bonn Medical Centre, Bonn, Germany, Biotest AG, 63303 Dreieich, Germany;ProBioGen AG, 13086 Berlin, Germany;

关键词: Peribunyaviridae;    orthobunyavirus;    arbovirus;    insect-specific virus;    mosquito;    Mexico;   

DOI  :  10.3390/v11090832

来源: DOAJ

【 摘 要 】

The Peribunyaviridae family contains the genera Orthobunyavirus, Herbevirus, Pacuvirus, and Shangavirus. Orthobunyaviruses and pacuviruses are mainly transmitted by blood-feeding insects and infect a variety of vertebrates whereas herbeviruses and shangaviruses have a host range restricted to insects. Here, we tested mosquitoes from a tropical rainforest in Mexico for infections with peribunyaviruses. We identified and characterized two previously unknown viruses, designated Baakal virus (BKAV) and Lakamha virus (LAKV). Sequencing and de novo assembly of the entire BKAV and LAKV genomes revealed that BKAV is an orthobunyavirus and LAKV is likely to belong to a new genus. LAKV was almost equidistant to the established peribunyavirus genera and branched as a deep rooting solitary lineage basal to herbeviruses. Virus isolation attempts of LAKV failed. BKAV is most closely related to the bird-associated orthobunyaviruses Koongol virus and Gamboa virus. BKAV was successfully isolated in mosquito cells but did not replicate in common mammalian cells from various species and organs. Also cells derived from chicken were not susceptible. Interestingly, BKAV can infect cells derived from a duck species that is endemic in the region where the BKAV-positive mosquito was collected. These results suggest a narrow host specificity and maintenance in a mosquito−bird transmission cycle.

【 授权许可】

Unknown