Genetic, Morphological and Antigenic Relationships between Mesonivirus Isolates from Australian Mosquitoes and Evidence for Their Horizontal Transmission

Viruses

Wenjun Liu¹  JoanneR. Kizu¹  Christina Hoare¹  Babak Shaban²  PenelopeJ. Gauci³  StephenL. Doggett⁴  John Haniotis⁴  AndrewF.van den Hurk⁵  Sonja Hall-Mendelin⁵  David Warrilow⁵  BreeannaJ. McLean⁶  Jody Hobson-Peters⁶  NataleeD. Newton⁶  Daniel Watterson⁶  Devina Paramitha⁶  AgatheM. G. Colmant⁶  CaitlinA. O’Brien⁶  Emma Ledger⁶  Helle Bielefeldt-Ohmann⁶  RoyA. Hall⁶  CherylA. Johansen⁷ 

[1] Australian Defence Force Malaria and Infectious Disease Institute, Gallipoli Barracks, Enoggera, 4051 Queensland, Australia;Australian Genome Research Facility Ltd., Parkville, 3050 Victoria, Australia;Defence Science & Technology Group, Fisherman’s Bend, 3207 Victoria, Australia;NSW Health Pathology-ICPMR Westmead, Medical Entomology, Westmead Hospital, Westmead, 2145 New South Wales, Australia;Public Health Virology, Queensland Health Forensic and Scientific Services, 4108 Queensland, Australia;School of Chemistry and Molecular Biosciences, University of Queensland, St Lucia, 4072 Queensland, Australia;School of Pathology and Laboratory Medicine, WA, 6009 Nedlands, Western Australia, Australia;

关键词: mesonivirus;    nidovirus;    insect-specific virus;    monoclonal antibody;    FTA card;   

DOI  :  10.3390/v12101159

来源: DOAJ

【 摘 要 】

The Mesoniviridae are a newly assigned family of viruses in the order Nidovirales. Unlike other nidoviruses, which include the Coronaviridae, mesoniviruses are restricted to mosquito hosts and do not infect vertebrate cells. To date there is little information on the morphological and antigenic characteristics of this new group of viruses and a dearth of mesonivirus-specific research tools. In this study we determined the genetic relationships of recent Australian isolates of Alphamesonivirus 4 (Casuarina virus—CASV) and Alphamesonivirus 1 (Nam Dinh virus—NDiV), obtained from multiple mosquito species. Australian isolates of NDiV showed high-level similarity to the prototype NDiV isolate from Vietnam (99% nucleotide (nt) and amino acid (aa) identity). Isolates of CASV from Central Queensland were genetically very similar to the prototype virus from Darwin (95–96% nt and 91–92% aa identity). Electron microscopy studies demonstrated that virion diameter (≈80 nm) and spike length (≈10 nm) were similar for both viruses. Monoclonal antibodies specific to CASV and NDiV revealed a close antigenic relationship between the two viruses with 13/34 mAbs recognising both viruses. We also detected NDiV RNA on honey-soaked nucleic acid preservation cards fed on by wild mosquitoes supporting a possible mechanism of horizontal transmission between insects in nature.

【 授权许可】

Unknown