Immobilized Enzymes in Biosensor Applications

Materials

Moonil Kim¹  HoangHiep Nguyen²  SunHyeok Lee²  UiJin Lee²  CesarD. Fermin³ 

[1] Sciences, Tuskegee University, Tuskegee, AL 36830, USA;BioNanotechnology Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), 125 Gwahangno, Yuseong-Gu, Daejeon 34141, Korea;;Department of Biology, College of Arts &

关键词: enzyme;    immobilization;    biosensor;    immobilized enzyme;    enzyme modification;   

DOI  :  10.3390/ma12010121

来源: DOAJ

【 摘 要 】

Enzyme-based biosensing devices have been extensively developed over the last few decades, and have proven to be innovative techniques in the qualitative and quantitative analysis of a variety of target substrates over a wide range of applications. Distinct advantages that enzyme-based biosensors provide, such as high sensitivity and specificity, portability, cost-effectiveness, and the possibilities for miniaturization and point-of-care diagnostic testing make them more and more attractive for research focused on clinical analysis, food safety control, or disease monitoring purposes. Therefore, this review article investigates the operating principle of enzymatic biosensors utilizing electrochemical, optical, thermistor, and piezoelectric measurement techniques and their applications in the literature, as well as approaches in improving the use of enzymes for biosensors.

【 授权许可】

Unknown