期刊论文详细信息
Micromachines
Continuous-Flow Cell Dipping and Medium Exchange in a Microdevice using Dielectrophoresis
Bashar El-Khasawneh1  Waqas Waheed1  Anas Alazzam1  Falah Alhammadi1 
[1] Mechanical Engineering Department, Khalifa University of Science and Technology, Abu Dhabi 127788, UAE;
关键词: dipping;    medium exchange;    dielectrophoresis;    microfluidics;    microchannel;    parametric studies;    blood cells;   
DOI  :  10.3390/mi9050223
来源: DOAJ
【 摘 要 】

Medium exchange is the process of changing the suspension medium of cells/particles, and has applications in washing, surface modifications, nutrient replenishment, or simply changing the environment of the target entities. Dipping involves diverting the path of target cells in the carrying fluid to immerse them in another fluid for a short duration, and pushing them again into the original medium. In this paper, a simple microfluidic platform is introduced that employs dielectrophoresis to achieve medium exchange and dipping of micro-objects in a continuous manner. The essential feature of the platform is a microchannel that includes two arrays of microelectrodes that partly enter the bottom surface from both sides. In the first step, numerous finite element-based parametric studies are carried out to obtain the optimized geometrical and operational parameters ensuring successful dipping and medium exchange processes. The results of those studies are utilized to fabricate the platform using standard photolithography techniques. The electrodes are patterned on a glass substrate, while the channel, made out of polydimethylsiloxane, is bonded on top of the glass. Trajectories of blood cells from numerical studies and experimentations are reported, and both results exhibited close agreement.

【 授权许可】

Unknown   

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