Constitutive Cytomorphologic Features of Medullary Thyroid Carcinoma Using Different Staining Methods

Diagnostics

Mitsuyoshi Hirokawa¹  Ayana Suzuki¹  Shinya Satoh²  Chan Kwon Jung³  Chiung-Ru Lai⁴  Jen-Fan Hang⁴  Kennichi Kakudo⁵  Shipra Agarwal⁶  Deepali Jain⁶  Chien-Chin Chen⁷  Somboon Keelawat⁸  Tikamporn Jitpasutham⁸  Yun Zhu⁹  Andrey Bychkov^1,10  Min En Nga^1,11  Noel Chia^1,11  Hee Young Na^1,12  So Yeon Park^1,12  Weiwei Li^1,13  Zhiyan Liu^1,14  Chih-Yi Liu^1,15  Priyanthi Kumarasinghe^1,16  Dilini Gunawardena^1,16 

[1] Department of Diagnostic Pathology and Cytology, Kuma Hospital, Kobe 650-0011, Japan;Department of Endocrine Surgery, Yamashita Thyroid and Parathyroid Clinic, Fukuoka 812-0034, Japan;Department of Hospital Pathology, College of Medicine, The Catholic University of Korea, Seoul 06591, Korea;Department of Pathology and Laboratory Medicine, Taipei Veterans General Hospital, Taipei 112, Taiwan;Department of Pathology and Thyroid Disease Center, Izumi City General Hospital, Izumi, Osaka 594-0073, Japan;Department of Pathology, All India Institute of Medical Sciences, New Delhi 110029, India;Department of Pathology, Ditmanson Medical Foundation Chia-Yi Christian Hospital, Chiayi 600, Taiwan;Department of Pathology, Faculty of Medicine, Chulalongkorn University, Bangkok 10330, Thailand;Department of Pathology, Jiangsu Institution of Nuclear Medicine, Wuxi 214063, China;Department of Pathology, Kameda Medical Center, Kamogawa, Chiba 296-8602, Japan;Department of Pathology, National University Hospital, Singapore 119074, Singapore;Department of Pathology, Seoul National University Bundang Hospital, Seoul National University College of Medicine, Seongnam 13620, Korea;Department of Pathology, Shandong University School of Basic Medical Sciences, Jinan 250012, China;Department of Pathology, Shanghai Jiao Tong University Affiliated Sixth People’s Hospital, Shanghai 200233, China;Division of Pathology, Sijhih Cathay General Hospital, New Taipei City 221, Taiwan;School of Pathology & Laboratory Medicine, University of Western Australia, Perth, WA 6009, Australia;

关键词: cytology;    fine-needle aspiration;    medullary thyroid carcinoma;    sensitivity;    specificity;    thyroid;   

DOI  :  10.3390/diagnostics11081396

来源: DOAJ

【 摘 要 】

(1) Background: Accurate preoperative identification of medullary thyroid carcinoma (MTC) is challenging due to a spectrum of cytomorphologic features. However, there is a scarcity of studies describing the cytomorphologic features as seen on fine-needle aspiration (FNA) smears prepared using different staining methods. (2) Methods: We performed a retrospective study on MTC cases with available FNA slides from 13 hospitals distributed across 8 Asia-Pacific countries. The differences in the constitutive cytomorphologic features of MTC with each cytopreparatory method were recorded. A comparative analysis of cytologic characteristics was carried out with appropriate statistical tests. (3) Results: Of a total of 167 MTC samples retrospectively recruited, 148 (88.6%) were interpreted as MTC/suspicious for MTC (S-MTC). The staining methods used were Papanicolaou, hematoxylin-eosin, and Romanowsky stains. Seven out of the eleven cytologic criteria can be readily recognized by all three cytopreparatory methods: high cellularity, cellular pleomorphism, plasmacytoid cells, round cells, dyshesive cells, salt-and-pepper chromatin, and binucleation or multinucleation. An accurate diagnosis was achieved in 125 (84.5%) of the 148 samples whose FNAs exhibited five or more atypical features. Conclusions: The present work is the first study on MTC to compare the morphological differences among the cytologic staining techniques. We investigated the constitutive features and the reliability of diagnostic parameters. A feasible scoring system based upon cytomorphologic data alone is proposed to achieve a high degree of diagnostic accuracy.

【 授权许可】

Unknown