Genomic and Transcriptomic Landscape of Tumor Clonal Evolution in Cholangiocarcinoma

Frontiers in Genetics

Fang-E Liu¹  Song Lin²  Jing Zhao²  Huqing Zhang²  Geng Chen²  Xi Hu²  Zhixiong Cai²  Xiaolong Liu³  Xiuqing Dong³ 

[1] Department of Nursing, School of Medicine, Xi’an Peihua University, Xi’an, China;School of Life Sciences and Technology, Xi’an Jiaotong University, Xi’an, China;The United Innovation of Mengchao Hepatobiliary Technology Key Laboratory of Fujian Province, Mengchao Hepatobiliary Hospital of Fujian Medical University, Fuzhou, China;

关键词: cholangiocarcinoma;    clonal evolution;    sequencing;    transcriptome;    genome;   

DOI  :  10.3389/fgene.2020.00195

来源: DOAJ

【 摘 要 】

Cholangiocarcinoma remained a severe threat to human health. Deciphering the genomic and/or transcriptomic profiles of tumor has been proved to be a promising strategy for exploring the mechanism of tumorigenesis and development, which could also provide valuable insights into Cholangiocarcinoma. However, little knowledge has been obtained regarding to how the alteration among different omics levels is connected. Here, using whole exome sequencing and transcriptome sequencing, we performed a thorough evaluation for the landscape of genome and transcriptome in cholangiocarcinoma and illustrate the alteration of tumor on different biological levels. Meanwhile, we also identified the clonal structure of each included tumor sample and discovered different clonal evolution patterns related to patients’ survival. Furthermore, we extracted subnetworks that were greatly influenced by tumor clonal/subclonal mutations or transcriptome change. The topology relationship between genes affected by genomic/transcriptomic changes in biological interaction networks revealed that alteration of genome and transcriptome was highly correlated, and somatic mutations located on important genes might affect the expression of numerous genes in close range.

【 授权许可】

Unknown