Brains in Metamorphosis: Temporal Transcriptome Dynamics in Hatchery-Reared Flatfishes

Biology

Laura Guerrero-Peña¹  Paula Suarez-Bregua¹  Josep Rotllant¹  Luis Méndez-Martínez¹  Juan J. Tena²  Juan A. Rubiolo³  Pablo García-Fernández⁴  Ricardo Tur⁴ 

[1] Aquatic Biotechnology Lab., Institute of Marine Research, Spanish National Research Council (IIM-CSIC), 36208 Vigo, Spain;Centro Andaluz de Biología del Desarrollo (CABD), CSIC-Universidad Pablo de Olavide, 41013 Sevilla, Spain;Facultad de Ciencias Bioquímicas y Farmacéuticas-Centro Científico y Tecnológico Acuario del Río Paraná, Universidad Nacional de Rosario, Rosario S2002LRK, Argentina;Nueva Pescanova Biomarine Center, S.L., 36980 O Grove, Spain;

关键词: turbot;    metamorphosis;    brain;    RNA;    sequencing;    transcriptome;   

DOI  :  10.3390/biology10121256

来源: DOAJ

【 摘 要 】

Metamorphosis is a captivating process of change during which the morphology of the larva is completely reshaped to face the new challenges of adult life. In the case of fish, this process initiated in the brain has traditionally been considered to be a critical rearing point and despite the pioneering molecular work carried out in other flatfishes, the underlying molecular basis is still relatively poorly characterized. Turbot brain transcriptome of three developmental stages (pre-metamorphic, climax of metamorphosis and post-metamorphic) were analyzed to study the gene expression dynamics throughout the metamorphic process. A total of 1570 genes were differentially expressed in the three developmental stages and we found a specific pattern of gene expression at each stage. Unexpectedly, at the climax stage of metamorphosis, we found highly expressed genes related to the immune response, while the biological pathway enrichment analysis in pre-metamorphic and post-metamorphic were related to cell differentiation and oxygen carrier activity, respectively. In addition, our results confirm the importance of thyroid stimulating hormone, increasing its expression during metamorphosis. Based on our findings, we assume that immune system activation during the climax of metamorphosis stage could be related to processes of larval tissue inflammation, resorption and replacement, as occurs in other vertebrates.

【 授权许可】

Unknown