Prevalence, Virulence Feature, Antibiotic Resistance and MLST Typing of Bacillus cereus Isolated From Retail Aquatic Products in China

Frontiers in Microbiology

Juan Wang¹  Hui Guo³  Shi Wu³  Jumei Zhang³  Shubo Yu³  Moutong Chen³  Ying Li³  Ying Zhang³  Mengfei Chen³  Haiyan Zeng³  Rui Pang³  Qinghua Ye³  Yu Ding³  Junhui Zhang³  Shuhong Zhang³  Qingping Wu³  Pengfei Yu³  Liang Xue³  Huan Zhou³ 

[1] College of Food Science, South China Agricultural University, Guangzhou, China;Department of Food Science and Technology, Institute of Food Safety and Nutrition, Jinan University, Guangzhou, China;State Key Laboratory of Applied Microbiology Southern China, Guangdong Provincial Key Laboratory of Microbial Safety and Health, Guangdong Open Laboratory of Applied Microbiology, Guangdong Institute of Microbiology, Guangzhou, China;

关键词: aquatic products;    Bacillus cereus;    prevalence;    MLST;    multi-drug resistance;   

DOI  :  10.3389/fmicb.2020.01513

来源: DOAJ

【 摘 要 】

Bacillus cereus is one of the most important foodborne pathogenic microorganisms, which can lead to gastrointestinal and non-gastrointestinal diseases. However, the potential risk of B. cereus in aquatic products in China has not been comprehensively evaluated yet. In this study, a total of 860 aquatic samples from three types of retail aquatic products were collected from 39 major cities in China from 2011 to 2016. The contamination, distribution of virulence genes, antibiotic resistance and genetic diversity of B. cereus isolates were measured and analyzed. Of all the samples, 219 (25.47%) were positive for B. cereus and 1.83% (4/219) of the samples had contamination levels of more than 1,100 most probable number (MPN)/g. Different isolates had virulence potential, within which 59.6% (164/275) contained all three kinds of enterotoxin genes (nhe, hbl, and cytK-2) and 5.1% (14/275) possessed cereulide encoding gene cesB. The antimicrobial resistance profiles revealed the universal antibiotic resistance to rifampin and most β-lactams, suggesting the necessity to continuously monitor the antibiotic resistance of B. cereus in aquatic products and to control drug use in aquaculture. In sum, our study indicates the potential hazards of B. cereus isolated from aquatic products to customers and may provide a reference for clinical treatment caused by B. cereus.

【 授权许可】

Unknown