Improving Magnetofection of Magnetic Polyethylenimine Nanoparticles into MG-63 Osteoblasts Using a Novel Uniform Magnetic Field

Nanoscale Research Letters

Yong Zhang¹  Chaode Cen²  Lina Xie³  Jun Wu³  Xin Zhang³  Cong Luo³  Ming Li³  Tongchuan He⁴  Yang Bi⁵  Tingyu Li⁵  Xiaolan Yang⁶ 

[1] Department of Gynaecology, The First People’s Hospital of Guiyang;Department of Orthopedics, Guizhou Provincial Orthopedics Hospital;Department of Orthopedics, Laboratory of Orthopedic Biomaterials, Children’s Hospital of Chongqing Medical University;Laboratory of Molecular Oncology, Department of Surgery/Orthopedics Center, The University of Chicago Medical Center;Ministry of Education Key Laboratory of Child Development and Disorders, Chongqing Engineering Research Center of Stem Cell Therapy, China International Science and Technology Cooperation base of Child Development and Critical Disorders;Ministry of Education Key Laboratory of Clinical Diagnostics, Department of Chemistry, Chongqing Medical University;

关键词: Magnetofection;    Magnetic nanoparticles;    Uniform magnetic field;    Polyethylenimine;    Non-viral gene delivery;   

DOI  :  10.1186/s11671-019-2882-5

来源: DOAJ

【 摘 要 】

Abstract This study aimed to improve the magnetofection of MG-63 osteoblasts by integrating the use of a novel uniform magnetic field with low molecular weight polyethylenimine modified superparamagnetic iron oxide nanoparticles (PEI-SPIO-NPs). The excellent characteristics of PEI-SPIO-NPs such as size, zeta potential, the pDNA binding and protective ability were determined to be suitable for gene delivery. The novel uniform magnetic field enabled polyethylenimine-modified superparamagnetic iron oxide nanoparticles/pDNA complexes (PEI-SPIO-NPs/pDNA complexes) to rapidly and uniformly distribute on the surface of MG-63 cells, averting local transfection and decreasing disruption of the membrane caused by the centralization of positively charged PEI-SPIO-NPs, thereby increasing the effective coverage of magnetic gene carriers during transfection, and improving magnetofection efficiency. This innovative uniform magnetic field can be used to determine the optimal amount between PEI-SPIO-NPs and pDNA, as well as screen for the optimal formulation design of magnetic gene carrier under the homogenous conditions. Most importantly, the novel uniform magnetic field facilitates the transfection of PEI-SPIO-NPs/pDNA into osteoblasts, thereby providing a novel approach for the targeted delivery of therapeutic genes to osteosarcoma tissues as well as a reference for the treatment of other tumors.

【 授权许可】

Unknown