Biofoam of Spittlebug, Poophilus costalis (Walker): Preferential Sites, Temperature Regulation, Chemical Composition and Antimicrobial Activity

Insects

Balakrishnan Saranya¹  Kitherian Sahayaraj¹  Koilraj Madasamy¹  Samy Sayed²  LokoYêyinou Laura Estelle³ 

[1] Crop Protection Research Centre, Department of Zoology, St. Xavier’s College, Palayamkottai, Tirunelveli 627002, Tamil Nadu, India;Department of Science and Technology, University College-Ranyah, Taif University, B.O. Box 11099, Taif 21944, Saudi Arabia;Laboratory of Applied Entomology, National High School of Biosciences and Applied Biotechnologies (ENSBBA), National University of Sciences, Technologies, Engineering and Mathematics, Dassa-Zoumé BP 14, Benin;

关键词: spittlebug;    biofoam;    biochemistry;    composition;    host plants;    antimicrobials;   

DOI  :  10.3390/insects12040340

来源: DOAJ

【 摘 要 】

The foam produced by nymphs of Poophilus costalis on eleven different host plants belonging to eight families on St. Xavier’s College campus in India was studied over five months. The chemical composition and antimicrobial activity of these biofoams were investigated. The results revealed that P. costalis preferred Theporsia purpurea and Mimosa pudica for laying their eggs and producing foam, over the other tested plants. P. costalis produce their foam on either nodes or internodes on monocotyledons (30%) (p < 0.05), whereas on dicotyledons, they produce more foam on the stems (63.8%) than on the leaves (6.2%) (p < 0.01). The number of nymphs in each piece of foam from P. costalis varied from 1 to 3 (mean = 1.8 per plant). They produced their foam (5.7 to 45.2 cm) from the ground level on a plant. The length and breadth of a piece of foam ranged from 1.0 to 3.9 cm and 0.6 to 4.7 cm, respectively. The foam tended to be cooler than the environment. Qualitative profiling showed that the foam consists of carbohydrates, including maltose; trypsin; amino acids; protease. The foam was also analyzed using a spectrophotometer, Fourier transform infrared spectroscopy (FT-IR), gas chromatography–mass spectroscopy (GC-MS), and high-performance liquid chromatography (HPLC). The antimicrobial activity of the biofoam was the greatest against Staphylococcus aureus, the growth of which was reduced by 55.9 ± 3.9%, suggesting that the foam could be used as an antimicrobial product. However, no activities were observed against Fusarium oxysporum and Candida albicans.

【 授权许可】

Unknown