Sesamol Inhibited Ultraviolet Radiation-Induced Hyperpigmentation and Damage in C57BL/6 Mouse Skin

Antioxidants

Chien-Wei Hou¹  Chien-Yih Lin²  Yi-Jung Liu³  Kuo-Ching Wen³  Hsiu-Mei Chiang³  Ya-Jhen You³  Chin-Sheng Wu³  Po-Yuan Wu⁴ 

[1] Department of Biotechnology and Pharmaceutical Technology, Yuanpei University of Medical Technology, Hsinchu 30015, Taiwan;Department of Biotechnology, Asia University, Taichung 41354, Taiwan;Department of Cosmeceutics, China Medical University, Taichung 40402, Taiwan;Department of Dermatology, China Medical University Hospital, Taichung 40402, Taiwan;

关键词: sesamol;    melanogenesis;    tyrosinase;    tyrosinase-related protein-1;    microphthalmia-associated transcription factor;   

DOI  :  10.3390/antiox8070207

来源: DOAJ

【 摘 要 】

Melanin is synthesized through a series of oxidative reactions initiated with tyrosine and catalyzed by melanogenesis-related proteins such as tyrosinase, tyrosinase-related protein-1 (TRP-1), dopachrome tautomerase (TRP-2), and microphthalmia-associated transcription factor (MITF). Our previous study demonstrated that sesamol inhibited melanin synthesis through the inhibition of the melanocortin 1 receptor (MC1R)/MITF/tyrosinase pathway in B16F10 cells. In this study, sesamol was applied to C57BL/6 mouse skin to understand its activity with respect to skin pigmentation. The results indicated that ultraviolet (UV) B-induced hyperpigmentation in the C57BL/6 mouse skin was significantly reduced by topical application of sesamol for 4 weeks. Sesamol reduced the melanin index and melanin content of the skin. In addition, sesamol elevated the brightness (L* value) of the skin. Sesamol also reduced UVB-induced hyperplasia of epidermis and collagen degradation in dermis. In immunohistochemical staining, topical application of sesamol reduced UVB-induced tyrosinase, TRP-1, TRP-2, and MITF expression in the epidermis of the skin. These results demonstrated that sesamol is a potent depigmenting agent in the animal model.

【 授权许可】

Unknown