Analysis of Human Faecal Host Proteins: Responsiveness to 10-Week Dietary Intervention Modifying Dietary Protein Intake in Elderly Males

Frontiers in Nutrition

Karl-Heinz Wagner¹  David Cameron-Smith²  Anders Sjödin³  Randall F. D'Souza⁵  Amber M. Milan⁶  Scott O. Knowles⁶  Nicole C. Roy⁷  Pankaja Sharma⁸  Nina Zeng⁸  Cameron J. Mitchell⁸  Farha Ramzan⁸  Sarah M. Mitchell⁸  Jessica L. Gathercole⁹  Ancy Thomas⁹  Anita J. Grosvenor⁹  Erin Lee⁹ 

[1] 0Department of Nutritional Sciences and Research Platform Active Ageing, University of Vienna, Vienna, Austria;1Agency for Science, Technology, and Research, Singapore Institute for Clinical Sciences, Singapore, Singapore;Department of Nutrition, Exercise, and Sports, Copenhagen University, Copenhagen, Denmark;Department of Nutrition, University of Otago, Dunedin, New Zealand;Discipline of Nutrition, University of Auckland, Auckland, New Zealand;Food, Nutrition, and Health Team, AgResearch, Auckland University, Auckland, New Zealand;High-Value Nutrition National Science Challenge, Auckland, New Zealand;Liggins Institute, University of Auckland, Auckland, New Zealand;Proteins and Metabolites Team, AgResearch, Lincoln, Christchurch, New Zealand;Riddet Institute, Massey University, Palmerston North, New Zealand;School of Kinesiology, University of British Columbia, Vancouver, BC, Canada;

关键词: faeces;    dietary protein;    host proteins;    gastrointestinal health;    proteomics;   

DOI  :  10.3389/fnut.2020.595905

来源: DOAJ

【 摘 要 】

Faecal proteomics targeting biomarkers of immunity and inflammation have demonstrated clinical application for the identification of changes in gastrointestinal function. However, there are limited comprehensive analyses of the host faecal proteome and how it may be influenced by dietary factors. To examine this, the Homo sapiens post-diet proteome of older males was analysed at the completion of a 10-week dietary intervention, either meeting the minimum dietary protein recommendations (RDA; n = 9) or twice the recommended dietary allowance (2RDA, n = 10). The host faecal proteome differed markedly between individuals, with only a small subset of proteins present in ≥ 60% of subjects (14 and 44 proteins, RDA and 2RDA, respectively, with only 7 common to both groups). No differences were observed between the diet groups on the profiles of host faecal proteins. Faecal proteins were detected from a wide range of protein classes, with high inter-individual variation and absence of obvious impact in response to diets with markedly different protein intake. This suggests that well-matched whole food diets with two-fold variation in protein intake maintained for 10 weeks have minimal impact on human faecal host proteins.

【 授权许可】

Unknown